温度对中华稻蝗小分子热休克蛋白基因表达的影响  

Effects of temperature on the expression patterns of Ocs HSP genes in Oxya chinensis

作　　者：史学凯[1,2,3] 寇利花 张育平[4] 马恩波[1,3] 张建珍[1,3] 吴海花[1,3] SHI Xue-Kai KOU Li-Hua ZHANG Jian-Zhen ZHANG Yu-Ping MA En-Bo WU Hai-Hua(Institute of Applied Biology, Shanxi University, Taiyuan 030006, China College of Life Science, Shanxi University Taiyuan 030006, China Biology Department, Taiyuan Normal University, Taiyuan 030619, China Shanxi Key Laboratory of Integrated Pest Management in Agriculture, Taiyuan 030006, China)

机构地区：[1]山西大学应用生物学研究所,太原030006 [2]山西大学生命科学学院,太原030006 [3]农业有害生物综合治理山西省重点实验室,太原030006 [4]太原师范学院生物系,太原030619

出　　处：《应用昆虫学报》2016年第6期1267-1273,共7页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金项目(31201548);山西省自然科学基金项目(2013011028-3)

摘　　要：【目的】小分子热休克蛋白(s HSPs)能够被环境胁迫所诱导,不同温度可诱导中华稻蝗体内sHSPs基因表达。【方法】本文采用实时荧光定量PCR(RT-q PCR)技术,分析不同温度处理后中华稻蝗Oxya chinensis体内6个Ocs HSPs基因的表达。【结果】结果表明,OcHSP19.1、OcHSP19.8、OcHSP20.4和OcHSP20.7基因的表达水平在27、32和37℃相同处理时间没有显著变化,但其表达量均在42℃显著升高,尤其是在42℃处理后2 h表达量升高明显。OcHSP21.1和OcHSP23.8基因的表达水平在不同温度处理后无显著变化。多因素方差分析结果表明,基因与处理温度间具有显著的交互作用。【结论】由此可见,温度能够对中华稻蝗s HSPs基因的表达产生影响,但是,不同温度处理下各基因的表达模式存在差异。[Objectives] Small heat shock proteins （sHSPs） can be induced by environmental stress. Different temperatures can induce the expression of sHSPs genes in Oxya chinensis. [Methods] The effect of temperature on the mRNA expression patterns of six OcsHSP genes in fifth-instar nymphs of O. chinensis was analyzed using Real-time quantitative PCR. [Results] There was no significant difference in the expression levels of OcHSP19.1, OcHSP19.8, OcHSP20.4 and OcHSP20.7 at 27, 32 and 37℃, but expression levels significantly increased at 42℃, especially after 2 h at 42℃. There was no significant difference in the expression levels of OcHSP21.1 and OcHSP23.8 at different temperatures. Multiple factor analysis of variance indicated that there was a significant interaction between temperature and gene. [Conclusion] Temperature can affect the expression of sHSPs genes in O. chinensis but different genes respond differently to temperature.

关 键 词：中华稻蝗 小分子热休克蛋白 温度 实时荧光定量PCR 

分 类 号：S433.2[农业科学—农业昆虫与害虫防治]