翻白草水提物对2型糖尿病大鼠胰岛形态及功能的保护机制研究  

Protection Mechanism of Potentilla discolor Aqueous Extract on the Morphology and Function of Type 2 Diabetic Rats Islet

作　　者：丁海波[1] 郑宇栋 徐杏[1] 陈曦[1] 刘莲[1] 陈广[2] 黄江荣[1] 

机构地区：[1]长江大学医学院,荆州434023 [2]华中科技大学同济医学院附属同济医院中西医结合科,武汉430030

出　　处：《天然产物研究与开发》2016年第12期1896-1902,1982,共8页Natural Product Research and Development

基　　金：湖北省自然科学基金(2015CFB320);长江大学2014年大学生创新创业训练计划(104892014037);荆州市医疗卫生科技计划(2014-17)

摘　　要：观察翻白草水提液对2型糖尿病大鼠胰岛形态及功能保护作用及机制。采用高脂乳剂灌胃加低剂量链脲佐菌素,建立2型糖尿病模型。生化技术检测血糖和血脂,ELISA检测血胰岛素含量,HE染色观察动物胰腺形态,免疫组化检测胰岛素、FoxO1及其磷酸化的表达。结果翻白草水提物可降低2型糖尿病大鼠血糖和总TC,促进胰岛素释放。光镜下胰岛素表达增加,胰岛β细胞核内FoxO1表达减少,包浆中p-FoxO1表达增加。翻白草水提液通过降低FoxO1,增加FoxO1的磷酸化表达,调控胰腺β细胞数量和功能,对2型糖尿病大鼠胰腺细胞具有保护作用。Abstract： To observe the protective effect of Potentilla discolor aqueous extract for type 2 diabetic rats islet. Type 2 diabetes rat model was induced by fat emulsion formulation and small dose of streptozocin. Biochemical assay was applied to measure serum glucose, total cholesterol （TC） , high density lipoprotein （HDL） ,low density lipoprotein （LDL） and tri- glycerides （TG）. ELISA was applied to measure serum insulin levels. Pancreatic histology was examined by HE stai- ning. Immunohistoehemistry （IHC） was employed to examine Forkhead box O 1 （FoxO1） ,p-FoxO1 and insulin protein expression. P. discolor aqueous extract groups significantly decreased random blood glucose and TC levels,increased in- sulin expression, promoted the release of insulin, decreased the expression of FoxO1 protein in the nucleus, induced the phosphorylation of FoxO1 protein and its getting out of the nucleus. P. discolor aqueous extract played a protective role for type 2 diabetic rats islet cell. The mechanism may be related to decrease FoxO1 expression and increase p-FoxO1, which further increase the number and improve the function of β cells.

关 键 词：胰腺 翻白草水提液 2型糖尿病 保护作用 FOXO1 

分 类 号：R961.1[医药卫生—药理学]