无乳链球菌CP+Sip-FbsA偶联蛋白免疫学特性的研究  

The immune effect of Streptococcus agalactiae recombinant CP+Sip-FbsA fusion protein on S.agalactiae induced mouse mastitis

作　　者：杜琳[1] 吕天星[1] 赵红梅[1] 李松建[1] 周雪[1] 崔健[1] 郝永清[1] DU Lin LV Tian-xing ZHAO Hong-Mei LI Song-jian ZHOU Xue CUI Jian HAO Yong-qing(Inner Mongolia Agricultural University, College of Veterinary Medicine, Inner Mongolia Hohhot 010010, China)

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010010

出　　处：《中国预防兽医学报》2016年第12期976-980,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技支撑项目(2012BAD12B09-2)

摘　　要：为研究无乳链球菌荚膜多糖(CP)与表面免疫相关蛋白(Sip)和纤维蛋白原结合蛋白(FbsA)串联表达蛋白的偶联物CP+Sip-FbsA对无乳链球菌性奶牛乳房炎的免疫保护效果,本研究将纯化的CP+Sip-FbsA偶联物与铝盐佐剂混合,免疫泌乳期Wistar大鼠。通过ELISA方法进行血清效价检测和特异性抗体动态监测,以及T淋巴细胞增殖试验、流式细胞术、全血吞噬调理试验和大鼠乳腺攻毒保护试验对其免疫学特性进行研究。结果表明:与对照组相比,CP+Sip-FbsA免疫组血清中的抗体水平明显增高,T淋巴细胞增殖试验、流式细胞术、全血吞噬调理试验、大鼠乳腺攻毒保护试验显示CP+Sip-FbsA免疫组免疫效果高于其他免疫组和PBS对照组。本研究结果表明,CP+Sip-FbsA偶联物对无乳链球菌性乳房炎免疫效果较好,为进一步研究无乳链球菌亚单位疫苗提供了实验依据。The purpose of this study was to evaluate the immune effect of Streptococcus agalactiae capsula saccharides （Cp） conjugated recombinant surface immunogenic protein （SIP）- fibrinogen binding proteins A （FbsA） （CP＋Sip-FbsA） on S.agalactiae induced mastitis. The lactating wistar rats that were immunized by the CP＋Sip-FbsA emulsify with aluminum hydroxide. Then the humoral and cellular immunoresponses were evaluated. The results show that the IgG titers in the serum were significant higher in rats group immunized with CP＋Sip-FbsA than that in the control group. Moreover, the tests of T lymphocyte proliferation assay, flow cytometry, whole blood test, and rat mammary gland demonstrated that immunized group （CP＋Sip-FbsA） were better than control group （p〈0.05） This study provided the basis of further developing subunit vaccine against S.agalactiae induced mastitis.

关 键 词：无乳链球菌 CP+Sip-FbsA偶联物 奶牛乳房炎 

分 类 号：S852.61[农业科学—基础兽医学]