翅碱蓬对盐沼沉积物微生物生物量及β-氨氧化细菌群落的影响——以双台河口为例  

Effects of Suaeda heteroptera on microbial biomass and the community structure of β-ammonia-oxidizing bacteria in salt marsh sediments: The case of the Shuangtai estuary

作　　者：刘姣[1] 刘长发 李盛德[3] 李晋 陶韦 李璐瑶[1] 马悦欣[1] 

机构地区：[1]农业部海洋水产增养殖学重点开放实验室,大连116023 [2]辽宁省高校近岸海洋环境科学与技术重点实验室,大连116023 [3]大连海洋大学理学院,大连116023 [4]盘锦海洋与水产研究所,盘锦124010

出　　处：《生态学报》2016年第24期8081-8090,共10页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目(41171389);国家海洋公益性行业科研专项资助项目(201305043);辽宁省高等学校优秀科技人才支持计划资助项目(LR2013035)

摘　　要：为了解翅碱蓬植被对盐沼沉积物微生物的影响,于2013年7月、8月、9月和11月对双台河口裸滩和翅碱蓬植被沉积物(10—15 cm)微生物生物量碳(MBC)、微生物生物量氮(MBN)、16S rRNA基因丰度、潜在硝化速率、β-氨氧化细菌(β-AOB)丰度及群落进行了调查。结果表明,不同采样日期裸滩沉积物MBC、翅碱蓬沉积物β-AOB amo A丰度和两种生境潜在硝化速率没有显著差异;而翅碱蓬沉积物MBC、裸滩沉积物β-AOB amo A丰度、两种生境MBN和16S rRNA基因丰度呈现时间波动。当所有采样日期的数据结合分析时,翅碱蓬植被显著影响沉积物MBC、MBN、细菌16S rRNA基因丰度、潜在硝化速率和β-AOB amo A丰度。从裸滩和翅碱蓬沉积物获得的β-AOB序列属于Nitrosospira和Nitrosomonas,翅碱蓬植被对β-AOB群落结构和多样性均有一定的影响。研究结果有助于了解翅碱蓬湿地中微生物的作用,为盐沼生境的生态修复技术提供参考。microbial rate, and Shuangtai The goal of this study was to examine the impacts of sedimentary colonization by Suaeda heteroptera on benthic biomass carbon （MBC）, microbial biomass nitrogen （MBN）, 16S rRNA gene abundance, potential nitrification the richness and community structure of E-ammonia-oxidizing bacteria （β-AOB） in a salt marsh located in , China. Sediment samples （10--15 cm） were collected from a site colonized by S. heteroptera and from an adjacent unvegetated mudflat in July, August, September, and November of 2013. No significant differences in MBC were detected in unvegetated sediment among the various sampling dates, whereas MBC in vegetated sediment increased significantly in August and September compared with July （P 〈 0. 05 ）; overall, MBC in vegetated sediment was significantly higher than in unvegetated sediment （P 〈 0. 05）. A similar temporal trend was detected for MBN in the two sites, with higher values recorded in August than in July and November （P 〈 0. 05 ） ; in addition, MBN in vegetated sediment increased significantly in July, August, and September compared with MBN in unvegetated sediment （P 〈 0.05）. Bacterial 16S rRNA gene abundance, measured using a real-time quantitative PCR technique, was significantly higher in both habitats in July, August, and September than in November （P 〈 0. 05 ）, and was significantly higher in vegetated sediment in August, September, and November than in unvegetated sediment （P 〈 0.05 ）. No significant differences in potential nitrification rates were detected within the two habitats for any of the sampling dates, but potential nitrification rate increased significantly in vegetated sediment in August and September compared with unvegetated sediment （P 〈 0.05 ）. The abundance of β-AOB ammonia monooxygenase subunit A gene （amoA） in unvegetated sediment was significantly higher in August than in September and November （P 〈 0.05） , but no difference was detected in the abundance of β-AOB

关 键 词：翅碱蓬 微生物生物量 16S rRNA基因丰度 硝化作用 β-AOB 

分 类 号：Q948.122.3[生物学—植物学]