Establishment of Universal RT-PCR Detection Method for Duck Reovirus  

作　　者：Yongjuan WANG Weiyong ZUO Shanyuan ZHU Anping WANG Shuang WU Weiming HONG Hui LU 

机构地区：[1]Jiangsu Agfi-animal Husbandry Vocational College/Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High Tech Research,Taizhou 225300,China

出　　处：《Agricultural Biotechnology》2016年第6期28-30,共3页农业生物技术（英文版）

基　　金：Supported by the Twelfth Batch of"Top Talent Project"of Jiangsu Province(NY023);Jiangsu Provincial Natural Science Research Project(16KJB-23004)

摘　　要：This study was conducted to rapidly detect clinical infection condition of duck reovirus. A pair of specific primers was designed according to gene se- quence of σC protein of duck reovirus, and a specific RT-PCR detection method of duck reovirus was established with genome of duck reovirus as template. Differ- ent samples were collected from ducks infected by suspected reovirus in Jiangsu Province and subjected to PCR detection. The results showed that the established RT-PCR method could specifically amplify the 438 bp sequence of the conservative region of σC gene, and detec the DNA of duck rcovirus as low as 1gf, with a detection rate of 100%. The RT-PCR method could be used for rapid clinical diagnosis of duck reovirus.This study was conducted to rapidly detect clinical infection condition of duck reovirus. A pair of specific primers was designed according to gene se- quence of σC protein of duck reovirus, and a specific RT-PCR detection method of duck reovirus was established with genome of duck reovirus as template. Differ- ent samples were collected from ducks infected by suspected reovirus in Jiangsu Province and subjected to PCR detection. The results showed that the established RT-PCR method could specifically amplify the 438 bp sequence of the conservative region of σC gene, and detec the DNA of duck rcovirus as low as 1gf, with a detection rate of 100%. The RT-PCR method could be used for rapid clinical diagnosis of duck reovirus.

关 键 词：DUCK REOVIRUS RT-PCR DETECTION 

分 类 号：S858.32[农业科学—临床兽医学]