Effect of Echinacea Polysaccharide on Secretion of IL-1αmRNA by IEC-6 Injured by LPS  

作　　者：Yanying ZHANG Qiumei SHI Qinghui JIA Guisheng GAO Guangping GAO Xinhua SHAO 

机构地区：[1]Hebei Normal University of Science&Technology,Hebei Province Key Laboratory of Preventive Veterinary Medicine,Changli 066600,China [2]Xinhua Keji Veterinary Medicine Co.,Ltd.of Hebei Province,Luancheng 051430,China

出　　处：《Agricultural Biotechnology》2016年第6期35-36,43,共3页农业生物技术（英文版）

基　　金：Supported by Natural Science Foundation of China(31472230);Natural Science Foundation of Hebei Province(C2014407068);Fund from Science and Technology Department of Hebei Province(NO.14966610D)

摘　　要：[ Objective ] This study was conducted to investigate Echinacea polysaccharide （EPS） on expression of tumor necrosis factor （TNF） under injury of intestinal epithelial cells （IEC-6） by lipopolysaccharide （LPS）, so as to discuss the action mechanism of EPS to injured cells. [Method] Total DNA was extracted with TRIzon reagent, TNF-α mRNA was amplified, and the amplification products were subjected to agarose gel electrophoresis and imaging. [Result] 50 μg/ml EPS could partially inhibited the production of IL-α mRNA by IEC-6 under the stimulation of LPS, while the inhibition of 2（10 and 500 μg/ml EPS on the level of IL-1α mRNA gradually increased with the concentration increasing; and IEC-6 cells pretreated with 50,100,200 and 500 μg/ml EPS for 24 h and then stimulated by 10 μml IPS for 1 and 4 h were analyzed by RT-PCR method, and it was found that the expression of IL-α mRNA induced by LPS could be effectively inhibited by EPS, and the inhibition rate at 4 h was higher than that at 1 h. [ Conclusion] EPS could play its role of protecting intestinal mucosa by inhibiting the secretion of IL-1α mRNA by cells under the stimulation of LPS, and such inhibition effects of EPS had concentration dependency and time dependency.[ Objective ] This study was conducted to investigate Echinacea polysaccharide （EPS） on expression of tumor necrosis factor （TNF） under injury of intestinal epithelial cells （IEC-6） by lipopolysaccharide （LPS）, so as to discuss the action mechanism of EPS to injured cells. [Method] Total DNA was extracted with TRIzon reagent, TNF-α mRNA was amplified, and the amplification products were subjected to agarose gel electrophoresis and imaging. [Result] 50 μg/ml EPS could partially inhibited the production of IL-α mRNA by IEC-6 under the stimulation of LPS, while the inhibition of 2（10 and 500 μg/ml EPS on the level of IL-1α mRNA gradually increased with the concentration increasing; and IEC-6 cells pretreated with 50,100,200 and 500 μg/ml EPS for 24 h and then stimulated by 10 μml IPS for 1 and 4 h were analyzed by RT-PCR method, and it was found that the expression of IL-α mRNA induced by LPS could be effectively inhibited by EPS, and the inhibition rate at 4 h was higher than that at 1 h. [ Conclusion] EPS could play its role of protecting intestinal mucosa by inhibiting the secretion of IL-1α mRNA by cells under the stimulation of LPS, and such inhibition effects of EPS had concentration dependency and time dependency.

关 键 词：Echinacea pelysaccharide Lipopelysaccharide Intestinal epithelial cell Inhibition Protection IL-1αmRNA 

分 类 号：S853.7[农业科学—临床兽医学]