无机解磷真菌A2的分离鉴定及解磷条件的响应面优化  

Screening and Identification of Phosphate-solubilizing Fungil Strain A2 and Response Surface Optimization of Phosphate-solubilizing Conditions

作　　者：王雪[1] 刘颖[1] 辛雨[1] 刘太娟 刘玉艳[1] 王效莹[1] 姚淑敏[1] 

机构地区：[1]曲阜师范大学生命科学学院,山东省曲阜市273165

出　　处：《曲阜师范大学学报（自然科学版）》2016年第4期73-78,共6页Journal of Qufu Normal University(Natural Science)

基　　金：国家级大学生创新训练项目(201410446002);国家自然科学基金资助项目(31200400)

摘　　要：从土壤中分离筛选解磷菌,对所分离的45株解磷菌进行解磷效果测定,最终得到一株真菌A2菌株,其解磷效果优于其他解磷菌株,对A2菌株进行形态的初步鉴定,然后利用核糖体内转录间隔区(ITS)进行分子鉴定,结果表明该菌株与黑曲霉Aspergillus niger BAM122菌株的ITS相似度达到99%,所以确定该菌为黑曲霉.利用单因素实验和响应面法对其解磷能力以及解磷的条件进行研究,最终得到一最佳解磷培养条件:葡萄糖3%,硫酸铵0.8%,初始pH=6,温度28℃,摇床转速170rpm,培养7天,解磷效果最好.Various phosphate-solubilizing microbe strains were isolated from the soil.After many times of screening and purification,a strain A2 with stronger phosphate-solubilizing capability was obtained,which belonged to Aspergillus genus,as identified by morphological and electron microscope and internal transcribed spacer（ITS）.Using single factor experiment and response surface method,the conditions of the phosphate-solubilizing ability was studied,the best culture conditions was 3% glucose,ammonium sulphate was 0.8%,initial pH was equal 6,temperature was 28℃,the shaking table speed was 170 rpm,and cultivation was time 7days.

关 键 词：解磷菌 ITS 黑曲霉 响应面 

分 类 号：Q939.9[生物学—微生物学]