顶空固相微萃取-气质联用技术结合内标法测定液体乳游离中链脂肪酸  

Determination of Liquid Milk by Headspace Solid-Phase Microextraction and Gas Chromatography Mass Spectrometry with Internal Standard

作　　者：刘景[1] 苏米亚[1] 徐致远[1] 游春萍[1] 刘振民[1] LIU Jing SU Miya XU Zhiyuan YOU Chunping LIU Zhenmin(State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy and Food Co. Ltd., Shanghai 200436, Chin)

机构地区：[1]光明乳业股份有限公司乳业研究院,上海乳业生物工程技术研究中心,乳业生物技术国家重点实验室,上海200436

出　　处：《乳业科学与技术》2016年第6期16-19,共4页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：“十二五”国家科技支撑计划项目(2012BAD12B08;2013BAD18B02);上海市科委项目(16DZ2280600);上海市闵行区重大产业技术攻关项目(2015MH093)

摘　　要：原料乳和乳制品中主要的游离中链脂肪酸(己酸、辛酸、癸酸)由于具有强烈的风味特征,是影响液体乳质量安全的重要化合物。实验采用顶空固相微萃取-气相色谱质谱法(headspace solid-phase micro extraction and gas chromatography mass spectrometry,HS-SPME-GC/MS)联用技术结合内标法定量的测定液体乳中游离中链脂肪酸的方法。结果表明:此测定方法准确度高、操作简单、萃取效率高。The major free middle-chain fatty acids （hexanoic acid, octanoic acid, decanoic acid） in raw milk and dairy products are regarded as important compounds that affect liquid milk quality and safety due to their strong flavor characteristics. This article provides an analytical method using headspace solid phase microextraction and gas chromatography-mass spectrometry （HS-SPME-GC-MS） with an internal standard for the quantitative determination of free middle-chain fatty acids in liquid milk. The method was verified as an approach with high accuracy, simple operation and high extraction efficiency.

关 键 词：乳 中链脂肪酸 顶空固相微萃取-气相色谱质谱法联用 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]