腺苷对脊髓背角NMDA受体的抑制性调节作用  

Inhibitory effects of adenosine on NMDA receptor in spinal cord dorsal horn

作　　者：杨云惠[1] 索占伟[1] 杨娴[1] 胡晓东[1] 

机构地区：[1]兰州大学药学院分子药理研究所,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2016年第6期15-19,共5页Journal of Lanzhou University（Medical Sciences）

基　　金：国家自然科学基金面上项目(31271186)

摘　　要：目的研究腺苷对脊髓背角N-甲基-D-天冬氨酸(NMDA)型谷氨酸受体的调节作用。方法制备SD大鼠离体脊髓片,在脊髓背角Ⅱ板层神经元上膜片钳全细胞记录NMDA受体介导的兴奋性突触后电流(EPSCs);提取脊髓背角突触后致密质(PSD)结构,蛋白质印迹法检查NMDA受体的含量。结果胞外灌流腺苷(50μmol/L)能够明显降低EPSCs的幅值(P<0.05),加速EPSCs的衰减(P<0.05);能够显著降低Glu N2B受体在兴奋性突触中的含量(P<0.05)。结论腺苷可能通过降低Glu N2B受体在突触的表达水平,抑制NMDA受体的痛觉突触传递。Objective To investigate the regulatory effect of adenosine on N- methyl- D- aspartate （NMDA） subtype glutamate receptor in spinal cord dorsal horn. Methods The spinal cord slices were prepared from SD rats. The excitatory postsynaptic currents （EPSCs） mediated by NMDA receptors were recorded in lamina II neurons under whole-cell patch clamp configuration. Postsynaptic density （PSD） was fractionated and immu- noblotted to analyze the synaptic expression of NMDA receptor. Results Extracellular perfusion of adenosine （50 μmol/L） significantly decreaseds the amplitude of EPSCs and accelerated the current decay （P 〈 0.05）. Meanwhile, adenosine noticeably reduced the synaptic contents GluN2B receptor （P 〈 0.05）. Conclusion Ade- nosine might blunt NMDA receptor-mediated nociceptive transmission by removing GluN2B receptor out of synapses.

关 键 词：腺苷 脊髓背角 N-甲基-D-天冬氨酸受体 疼痛 

分 类 号：R966[医药卫生—药理学]