AMHR2在人子宫内膜组织中的表达及相关研究  

The expression and correlation studies of Anti?Müllerian hormone receptor 2 in endometrium undergoing IVF

作　　者：刘磊[1] 芦小单[1] 林秀英[1] 孙牧男[1] 付建华[1] 谭岩[1] 方艳秋[1] LIU Lei LU Xiao-dan LIN Xiu-ying et al(Reproductive Medical Center, Jilin Province People＇s Hospital, Changchun 130021, China)

机构地区：[1]吉林省人民医院生殖医学中心,吉林长春130021

出　　处：《中国实验诊断学》2016年第11期1856-1858,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅项目(20160414021GH;20150414023GH;20150520038JH);吉林省卫计委项目(2013Q009)

摘　　要：目的探讨抗苗勒氏管激素Ⅱ型受体(AMHR2))在IVF-ET患者的子宫内膜组织着床窗口期的表达及意义。方法采用免疫组织荧光染色检测AMHR2在IVF-ET患者黄体期子宫内膜组织中的表达情况。分离培养原代子宫内膜间质细胞,用HCG处理细胞48h,荧光定量PCR检测HCG处理前后AMHR2基因表达情况;细胞化学染色检测HCG处理前后AMHR2蛋白表达情况。结果 AMHR2在黄体中期人子宫内膜组织表达;HCG处理后子宫内膜细胞中AMHR2的基因和蛋白水平均显著升高。结论 AMHR2在着床窗期子宫内膜间质细胞膜上表达,HCG可以增加其表达量,AMHR2可能作为预测子宫内膜容受性的参考指标。Objective To investigate the expression and significance of AMHR2 in the endometrium during the implantation window period of the patients with IVF-ET.Methods The expression of AMHR2 in the endometrium of IVF-ET patients during the phase of corpus phase was detected by immunofluorescence staining.The endometrial stromal cells were isolated and cultured,and the cultured cells were treated by hCG for48 h.The expression of AMHR2 was detected by method of PCR and Immunohistochemical analysis.Results The expression of AMHR2 were detected in endometrial implantation window,and the levels of AMHR2 gene and protein in endometrial cells were significantly increased after HCG treatment.Conclusion AMHR2 was detected in the endometrium during the implantation window of women,and level of AMHR2 were affected by HCG.AMHR2 might be a reference index to predict endometrial receptivity.

关 键 词：抗苗勒氏管激素Ⅱ型受体 子宫内膜容受性 体外受精移植技术 

分 类 号：R714.8[医药卫生—妇产科学] R446.6[医药卫生—临床医学]