人参皂苷Rg5对人食管癌细胞作用的研究  

Effect of ginsenoside Rg5 on human esophageal cancer cells

作　　者：梅志宏[1] 丁雅明[1] 李凡[2] 付学奇[3] 刘林林[1] 朱洪权[1] MEI Zhi-hong DING Ya-ming LI Fan et al(The Second Hospital of Jilin University, Changchun 130041, China)

机构地区：[1]吉林大学第二医院,吉林长春130041 [2]吉林大学基础医学院 [3]吉林大学生命科学院

出　　处：《中国实验诊断学》2016年第12期1982-1985,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金重大国际合作项目(项目编号:81320108025)

摘　　要：目的探索人参皂苷Rg5对人食管癌细胞Eca-109的作用及机制。方法利用不同浓度人参皂苷Rg5在体外对人食管癌Eca109细胞作用不同时间,采用CCK-8法检测细胞抑制率,Annexin V-FITC/PI双染色后流式细胞仪检测细胞凋亡率,PI单染法检测细胞周期。结果在浓度分别为50、100μmol/L的人参皂苷Rg5作用人食管癌细胞24h后出现明显形态学变化,同时处于S期及G2/M期细胞较对照组明显增多,分别为53.08%、25.84%和58.44%、31.48%,流式细胞仪检测早期凋亡率分别为11.45%、30.13%。结论人参皂苷Rg5能抑制食管癌Eca-109细胞增殖,其机制可能为增加细胞早期凋亡,引起细胞周期滞留。Objective To explore the effect and mechanism of ginsenoside Rg5 on human esophageal cancer cell line Eca-109.Methods The inhibitory rate of human esophageal cancer cell line Eca109 in vitro was measured by CCK-8 method.The apoptosis rate of cells was detected by flow cytometry after Annexin V-FITC/PI double staining.Cell cy-cle.Results After treated with ginsenoside Rg5 at concentrations of 50 and 100μmol/L for 24 h,the morphological changes were observed,and the cells in S phase and G2/M phase were significantly higher than those in the control group （53.08%,25.84%% and 58.44%,31.48% respectively（P〈0.05）.The early apoptotic rates were 11.45% and 30.13% respectively by flow cytometry（P〈0.05）.Conclusion Ginsenoside Rg5 can inhibit the proliferation of esoph-ageal cancer Eca-109 cells,and its mechanism may be to increase the early apoptosis of cells,causing cell cycle arrest.

关 键 词：人参皂苷Rg5 食管癌细胞Eca109 细胞周期 凋亡 

分 类 号：R285[医药卫生—中药学]