高效液相色谱法同时测定畜禽废水中4种四环素类抗生素  

Determination of Four Tetracycline Antibiotics in Swine Waste Water Using High-Performance Liquid Chromatography

作　　者：汤弘智 陈风春[1] 王鸿英[2] 张永伟[2] 王永颖 刘飞[1] 刘芳[1] 刘忠文[1] 高志贤[1] 周焕英[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所天津市环境与食品安全风险监控技术重点实验室,天津300050 [2]天津市畜牧兽医局

出　　处：《解放军预防医学杂志》2016年第6期910-912,共3页Journal of Preventive Medicine of Chinese People's Liberation Army

基　　金：天津市科技计划项目(No.16YFZCNC00730)

摘　　要：目的利用高效液相色谱-二极管阵列检测器,建立同时检测畜禽废水中土霉素、四环素、金霉素、强力霉素的方法。方法选用Waters SunF ire C18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.5%三氟乙酸(TFA)水溶液为流动相梯度洗脱,流速1.2ml/min,检测波长360nm,高效液相色谱仪测定。结果土霉素、四环素、金霉素和强力霉素4种抗生素质量浓度为0.1μg/ml^15.0μg/ml时有良好的线性关系,4种抗生素的检出限均为50ng/ml,方法回收率在83.82%~108.70%之间。结论高效液相色谱法方便、快速、准确,且重现性好,能够用于畜禽废水中四环素类抗生素的检测。Objective To establish a method for determination of four tetracycline antibiotics in swine waste water by HPLC. Methods After filtration and acid-digestion, 20 μl samples were injected into an HPLC system. Acetonitrile and 0.5% TFA solution were applied in gradient elution using HPLC method. The total running time was 15 minutes. Results The standard curve of four compounds presented a good linearity（ R2 〉0.999） in the range of 0.1 to 15.0 μg/ml. The detection limits were 50 ng/ml. The recovery ranged from 83.82% to 108.70% with the relative standard deviation less than 9.30%. Conclusion The proposed method presents many advantages, such as quick speed, user-friendliness and reproducibility.

关 键 词：高效液相色谱法 四环素类 畜禽废水 

分 类 号：O657[理学—分析化学] X832[理学—化学]