化积益肝煎对H22荷瘤小鼠抑瘤作用的实验研究  

Experimental Study of Huaji Yigan Decoction on H22 Cells-bearing Mice

作　　者：郑金玲[1] 张红[2] 吴庆连[1] 

机构地区：[1]大连医科大学附属第二医院中医康复科,辽宁大连116023 [2]大连医科大学附属第二医院中医科,辽宁大连116023

出　　处：《四川中医》2016年第11期45-48,共4页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:研究化积益肝煎对小鼠肝癌腹水瘤细胞系H22治疗的作用机制。方法:小鼠右腋下皮下种瘤造成实体瘤模型,将24只小鼠分为三组,生理盐水组、化积益肝煎组(中药组)和5-氟脲嘧啶组(西药组)各8只,用药14天后取瘤,称重计算抑瘤率,采用免疫组化技术检测C–erb B-2及Fasl的表达。结果:中药组、西药组大鼠瘤重显著低于对照组(P<0.05);中药组、西药组的抑瘤率分别为32.11%、38.61%;C–erb B-2及Fasl在三组中表达结果经秩和检验,与对照组比较,中药组C–erb B-2、Fasl表达减弱,差异均有统计学意义(P<0.05),而与西药组比较差异无统计学意义(P>0.05)。结论:化积益肝煎对肝癌H22细胞的增殖有明显的抑制作用,其作用机制与C–erb B-2、Fasl表达有关。Objective： To study the mechanism of using Huaji Yigan Decoction for treating ascites hepatoma cell line H22.Methods： After injecting ascites hepatoma cell line H22 ceils into their right oxter,24 mice were divided into three groups： the controlled group received treatment of normal solution,the treatment group treated by Huaji Yigan Decoction and the treatment group treated by 5-Fu.After 14 days inhibiting rate was accounted,C-erbB-2and Fasl were examined by using immunohistochemistry straining method in the tumor tissue.Results： The inhibiting rate was 32.11% in hepatoma cell by using Huaji Yigan Decoction.C-erbB-2and Fasl in the three groups were positive.One-factor analysis of variance manifest： the expression of C-erbB-2and Fasl was decreased in the treatment group.The difference between the two groups were significant（P〈0.05）.The comparison between Huaji Yigan Decoction group and 5-Fu（P〉0.05）,were not statistically significant.Conclusion： H22 cellsbearing mice are treated with Huaji Yigan Decoction can significantly inhibit H22 cells,and the mechanism may is relative to expressions of C-erbB-2and Fasl.

关 键 词：化积益肝煎 FASL C-ERBB-2 肝癌 H22荷瘤小鼠 

分 类 号：R285.5[医药卫生—中药学]