麝香促进肺腺癌细胞增殖及凋亡效果的体外实验研究  

Experimental Study on the Effect of Musk on Promoting Cell Proliferation and Apoptosis of Lung Adenocarcinoma Cells in Vitro

作　　者：马伟[1] 

机构地区：[1]青海大学附属医院心胸血管外科,青海西宁810000

出　　处：《四川中医》2016年第11期48-51,共4页Journal of Sichuan of Traditional Chinese Medicine

摘　　要：目的:分析麝香抑制肺腺癌细胞株GLC-82细胞增殖及促进凋亡的作用,为肺腺癌的抗肿瘤治疗提供新的研究方向。方法:采用四甲基偶氮唑蓝(MTT)法检测不同浓度麝香溶液处理GLC-82细胞后相应时间点的存活率,分别对其细胞形态学变化、细胞凋亡率进行观察,分析细胞增殖及凋亡状态变化。结果:MTT实验结果示,随着麝香溶液浓度的上升及处理时间的增加,GLC-82细胞存活率逐渐下降。倒置显微镜观察结果示,空白组肺腺癌GLC-82细胞贴壁生长,细胞间紧密连接,细胞形态呈舒展状,未见折光不均,细胞核较大且明显;200μg/m L麝香溶液处理后的GLC-82细胞体积减小、细胞间紧密连接消失,细胞核周围可见空泡样结构存在、核物质边集;麝香溶液浓度越高,GLC-82细胞核异常凝集、核固缩、凋亡小体形成等典型的细胞凋亡形态越为明显。流式细胞仪检测结果示,随着麝香溶液浓度的增加,细胞由早期凋亡为主转向晚期凋亡和继发性坏死。结论:麝香对肺腺癌细胞株GLC-82细胞增殖具有一定的抑制作用,且可促进GLC-82细胞凋亡,其抗肿瘤机理值得进一步深入研究。Objective： To analyze the effect of Musk on the proliferation and apoptosis of lung adenocarcinoma cell line GLC-82,and to provide a new research direction for the treatment of lung adenocarcinoma.Methods： Used methyl thiazolyl tetrazolium blue GLC-82 cells were treated with MTT method was used to detect different concentrations of musk solution at the same time survival rate,respectively on the morphological changes of the cells,cell apoptosis rate were observed.Analysis of cell proliferation and apoptosis of state changes.Results： MTT experimental results showed that the survival rate of GLC-82 cells decreased gradually with the increase of the concentration of musk solution and the increase of treatment time.Inverted microscope observation showed,the blank group,the lung adenocarcinoma GLC-82 cells adherent growth,tight junctions between cells,cell morphology was like stretch,no refraction was uneven.The nuclei were large and obvious; 200 UG / ml.The musk solution treatment of GLC-82 cell volume decreased and the intercellular tight junctions disappear,around the nucleus of the vacuole like structure,nuclear material edge set; musk solution concentration was high,GLC-82 cell abnormal agglutination,nuclear pyknosis,apoptotic body formation typical apoptotic morphology was more obvious.The results of flow cytometry showed that with the increase of the concentration of musk solution,the cell apoptosis and secondary necrosis were mainly from the early apoptosis.Conclusion： Musk has a certain inhibitory effect on the proliferation of lung adenocarcinoma cell line GLC-82,and can promote the apoptosis of GLC-82 cells.The anti-tumor mechanism is worthy of further study.

关 键 词：麝香 肺腺癌 细胞株GLC-82 细胞增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]