低温驯化对冬小麦叶绿素荧光特性及抗氧化酶活性的影响  

Effects of cold acclimation on chlorophyll fluorescence characteristics and antioxidant enzyme activity in winter wheat

作　　者：王秀田[1] 卢秋巍 苍晶[1] 包雨卓 孟德义 于晶[1] 徐庆华[1] 赵虎 李想[1] 相智也 谢波[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《植物生理学报》2016年第12期1959-1969,共11页Plant Physiology Journal

基　　金：国家自然科学基金(31471423);国家自然科学人才培养基金科研训练项目(J1210069);黑龙江省自然科学基金(C201418)~~

摘　　要：为探究不同抗寒性冬小麦品种不同生长发育期的光合特性,以强抗寒性冬小麦品种‘东农冬麦1号’(‘DN1’)和弱抗寒性冬小麦品种‘济麦22’(‘JM22’)为试验材料,在大田自然降温与室内模拟低温驯化条件下,从三叶期开始测定其叶绿素荧光参数、色素含量、抗氧化酶活性等相关生理指标,同时对自然生长条件下小麦幼苗编码光合相关蛋白的基因表达模式进行分析,探讨和揭示冬小麦叶绿素荧光特性对低温驯化的响应。结果表明:大田自然降温下,‘DN1’通过明显增加的NPQ、较高的色素含量和抗氧化酶活性以及较低的MDA含量,来适应温度变化对其光合特性的影响;而‘JM22’则对该时期温度变化不敏感,各项指标较为恒定;室内模拟低温驯化下,‘DN1’与自然条件下有类似的表现,且在昼夜温度4°C/0°C条件下驯化效果更明显。q PCR结果表明:两个小麦品种的叶绿素a/b结合蛋白基因(Ta CP3、Ta CP5),在测定的各时期均有较高表达,与色素含量变化相对应;参与光化学淬灭的基因Ta NPQ4‘DN1’均保持了较高的表达量,与其较高的NPQ变化相对应。综上表明,在该实验检测时期内强抗寒性‘DN1’能较早感受温度的降低而维持其光合系统的稳定,进而适应后期更低温度的胁迫。To explore the different photosynthetic characteristics of winter wheat in different growth and development period,two winter wheat cultivars freeze-tolerent ‘Dongnongdongmai 1＇（‘DN1＇） and freeze-sensitive ‘Jimai 22＇（‘JM22＇） were used in this study.Chlorophyll fluorescence parameters,chlorophyll content,MDA content,antioxidant enzyme activity were analyzed at the beginning of three-true leaf period under natural growth condition and low temperature indoors.Gene expression analysis of encoding related proteins of photosystem II in nature condition also was measured.The results are as follows： in natural conditions,‘DN1＇ adapt to temperature changes in chlorophyll fluorescence characteristics by a significant increase in NPQ,higher pigment content and antioxidant enzyme activity and lower MDA content; but ‘JM22＇ was insensitive to temperature and chlorophyll fluorescence indicators is still relatively constant.Under low temperature indoors,‘DN1＇ have similar performance to natural conditions and in 4°C/0°C（day/night） acclimation is more effective.q PCR results show： chlorophyll a/b-binding protein gene（Ta CP3,Ta CP5） have a higher expression of two wheat in each period,and corresponding changes of pigments; Ta NPQ4 involved in photochemical quenching have maintained a relatively high expression levels with the corresponding of higher NPQ in ‘DN1＇.In summary it shows that ‘DN1＇ can earlier feel the temperature decrease and maintain the stability of the photosynthetic system,then adapt to lower temperature stress of the late.

关 键 词：冬小麦 叶绿素荧光 抗氧化酶活性 叶绿素结合蛋白基因 

分 类 号：S512.11[农业科学—作物学]