18种披碱草属种质资源苗期抗旱性鉴定  

The Identification on Drought-Resistance of 18 Elymus L. germplasm Materials in Seeding Stage

作　　者：刘勇[1] 张永超[1] 魏小星[1] 周改娥 

机构地区：[1]青海省青藏高原优良牧草种质资源利用重点实验室,青海大学,青海省畜牧兽医科学院,西宁810016 [2]青海普兰生态科技有限责任公司,西宁810000

出　　处：《青海畜牧兽医杂志》2016年第6期16-23,共8页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：农业部行业专项“青藏高原社区特色生态畜牧业关键技术集成与示范”(201203010);“现代农业产业技术体系建设专项资金”(CARS-35-41);农业部“青藏高原牧草种质资源保护利用”项目(070401);青海大学中青年基金-环湖地区人工治沙植被作用下土壤酶及土壤养分变化规律研究

摘　　要：试验选取18份来自不同地区的披碱草属(Elymus L.)种质材料进行抗旱性研究,对牧草苗期进行连续干旱,并测定干旱第0d,5d,10d的细胞膜透性、丙二醛(MDA)、可溶性糖、脯氨酸和叶绿素的含量,并运用Fuzzy数学中隶属函数法进行抗旱性综合评判,比较18种材料的抗旱性强弱。结果表明,连续干旱胁迫下,披碱草属各牧草随着胁迫强度的增大和时间的延长叶绿素含量显著降低(P<0.05),且种间也具显著差异(P<0.05);而细胞膜透性、脯氨酸、丙二醛(MDA)、可溶性糖含量显著增加(P<0.05),且种间也具显著差异(P<0.05);经评定披碱草属18种牧草苗期抗旱性顺序为:I-1-1-21>I-1-5-63>09-202>I-1-17-5>09-037>5>I-1-5-39>I-1-5-67>I-1-1-26>09-050>3>2>I-1-5-44>4>I-1-5-37>I-1-1-35>I-1-5-12>I-1-1-5。Research on the dynamic variation of drought resistance in forages system is great importance for forageintroduction and selection, especially indrought areas. Taking eighteen Elymus L. germplasm materials from different ar-easas experimental materials, analyzed the dynamic variation of MDA, Content of chlorophyll, Relative electrical conduc-tivity rate, soluble sugar and protein in 0d,5d,10d after drought treatment. Using fuzzy mathematics method evaluatedthe drought resistance ability. The results indicated that relative electrical conductivity rate, MDA, soluble sugar andprotein contents of every herbage increased significantly along with the increasing drought stress and time extending （P〈 0. 05 ）, and there was significantly different between species （ P 〈 0.05 ）. The Content of chlorophyll contents of everyherbage had significant decline trend with the increasing drought stress and time extending （ P 〈 0. 05 ）, there was a sig-nificant difference between species （P 〈 0. 05 ）. At last, by using fuzzy mathematics method, the drought resistance a-bility of Elymus L. gramineous grass species tested was evaluated as follows ：I - 1 - 1 -21 〉 I - 1 -5 -63 〉 09 -202 〉I- ! - 17 -5 〉09 -037 〉5 〉I- 1 -5 -39 〉I- 1 -5 -67 〉 I- 1 - 1 -26 〉09 -050 〉3 〉2 〉 I - 1 -5 -44 〉4 〉 I -1-5-37〉I-1-1-35〉I -1-5-12〉I-1-1-5.

关 键 词：披碱草属牧草 抗旱性 细胞膜透性 可溶性糖 脯氨酸 丙二醛(MDA) 叶绿素 

分 类 号：S543.9[农业科学—作物学]