香连丸对化疗相关性腹泻小鼠生存时间与回肠组织中促炎因子的影响  

Effect of Xianglian Pill on Survival Time and Proinflammatory Cytokines in Mice with Chemotherapy-induced Diarrhea

作　　者：张新峰 乔翠霞[2] 程旭锋[3] 刘琦[3] 高启龙 王怀璋 杨峰 

机构地区：[1]郑州大学附属肿瘤医院中西医科,河南郑州450008 [2]河南省中医药研究院附属医院,河南郑州450008 [3]河南中医学院,河南郑州450008

出　　处：《辽宁中医杂志》2016年第12期2661-2663,I0020,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81603496;U1304822);河南省医学科技攻关计划项目(201304099);河南省科技攻关项目(162102310331);河南省基础与前沿技术研究(132300410042;152300410240)

摘　　要：目的:观察香连丸对化疗相关性腹泻(CID)小鼠生存时间、腹泻程度、肠道病理损伤、回肠组织中促炎细胞因子TNF-α和IFN-γ的影响,探讨其防治CID的可能机理。方法:采用腹腔注射伊立替康法制备CID裸鼠模型;小鼠随机分为正常组、模型组、洛哌丁胺组、香连丸组。观察裸鼠生存时间、腹泻情况、病理改变及回肠组织中TNF-α和IFN-γ表达情况。结果:与模型组比较,香连丸组生存时间延长(P<0.01),腹泻评分及小肠病理损伤程度均较明显降低(P<0.01),回肠组织中TNF-α、IFN-γ的含量均显著降低(P<0.05,P<0.01)。结论:香连丸对CID具有一定的防治作用,其机制可能与其下调回肠组织中促炎细胞因子TNF-α、IFN-γ的表达有关。Objective ：To observe the effect of Xianglian Pill on the survival time of mice, diarrhea, intestinal injury,TNF-α and IFN -γ in ileum tissue in mice with chemotherapy -induced diarrhea （CID） and explore its underlying mechanisms. Methods：The model of CID mice was set up by using intraperitoneal injection of irinotecan. Mice were randomly divided into normal group, model group, Loperamide group and Xianglian Pill group. The survival time, diarrhea, intestinal pathological changes, TNF - κ and IFN -γ in ileum of each group were evaluated. Results ： Compared with the model group, the survival time was extended （P 〈0. 01 ） and diarrhea score and ileum pathological degree of injury were obviously decreased （P 〈0. 01 ）. TNF -α and IFN -γ in ileum were significantly decreased （P 〈 0. 05, P 〈 0. 01 ） in the Xianglian Pill group. Conclusion ：Xianglian Pill has the therapeutic effect on CID and the mechanisms may be that it could lower the expression levels of TNF - α and IFN -γ in ileum.

关 键 词：香连丸 化疗相关性腹泻 生存时间 促炎细胞因子 

分 类 号：R285[医药卫生—中药学]