转化生长因子β1和细胞外信号调节激酶1/2在冠心病猝死早期心肌缺血中的表达及意义  

Expression and significance of TGF-β1 and ERK1/2 in the sudden death of coronary heart disease early myocardial ischemia

作　　者：章丽霞[1] 王宇 张丽华[1] 牛彩琴[1] 

机构地区：[1]川北医学院,四川南充637000 [2]平凉市妇幼保健医院,甘肃平凉744300

出　　处：《川北医学院学报》2016年第6期813-815,共3页Journal of North Sichuan Medical College

基　　金：四川省教育厅课题(12ZB225;14ZA0182)

摘　　要：目的:了解冠心病猝死(sudden coronary death,SCD)者心肌中TGF-β1和ERK1/2中的表达情况,探讨其对SCD诊断的意义。方法:采用免疫组织化学方法检测转化生长因子β1(transforming growth factor-β1,TGF-β1)和细胞外信号调节激酶1/2(extracellular signal-regulated kinase 1/2,ERK1/2)在30例SCD心肌样本(SCD组)和15例非心源性即刻死亡心肌样本(对照组)的表达情况。结果:SCD组心肌TGF-β1和ERK1/2的表达明显高于对照组,t值TGF-β1为19.807,ERK1/2为15.478,两种指标组间比较,差异均有统计学意义(P<0.01);TGF-β1和ERK1/2在SCD组中的表达呈正相关(r=0.642,P<0.01)。结论:心肌缺血激活TGF-β1/ERK1/2信号通路,使其在心肌中表达明显升高,检测TGF-β1和ERK1/2可为SCD的早期心肌缺血病理诊断提供客观指标。Objective：To observe the expressions of transforming growth factor-β1 （TGF-β1） and extracellular signal-regulated kinase 1/2 （ERK1/2） in the myocardium of patients of sudden coronary death and to explore the significance of SCD diagnosis. Methods ：The heart samples of thirty cases of sudden coronary death were chosen as experiment group while the heart samples of fifteen cases of non sudden coronary death were chosen as control group. Immunohistochemical staining was employed to examine the expressions of TGF-β1 and ERK1/2 in the myocardium. Results：The expressions of TGF-β1 and ERK1/2 in experiment group was significantly higher than those in control group,the t value of TGF-β1 was 19. 807,ERK1/2 was 15. 478, two indicators between two groups were compared,the differences were statistically significant （P 〈 0. 01 ） . The expressions of TGF-β1 positively related to ERK1/2 in SCD （r = 0. 64 ,P 〈 0.01 ）. Conclusion ：TGF-β1/ERK1/2 signaling pathway could participate in the process of apoptosis, the detection of the positive expressions of two indicators in the myocardium may provide an objective index for the early pathological diagnosis of SCD.

关 键 词：冠心病猝死 转化生长因子Β1 细胞外信号调节激酶1/2 免疫组织化学 

分 类 号：R541.4[医药卫生—心血管疾病]