甘肃黑蛋巢抗松梢枯病菌物质的分离纯化及抑菌活性  

ISOLATION AND PURIFICATION OF INHIBITORY SUBSTANCE FROM CYATHUS GANSUENSIS AND INHIBITION EFFECT OF THE SUBSTANCE AGAINST SPHAEROPSIS SAPINEA

作　　者：杨斌[1] 余静[2] 朱向东[1] 周彤燊[1] 

机构地区：[1]西南林学院生物技术室,昆明650224 [2]成都军区军事医学研究所,昆明650224

出　　处：《菌物系统》2002年第3期388-393,共6页Mycosystema

基　　金：国家自然科学基金; 云南省青年基金资助项目

摘　　要：采用MS培养基培养甘肃黑蛋巢Cyathus gansuensis,以生测为导向,乙酸乙酯和氯仿从甘肃黑蛋巢MS培养液中萃取出抗菌活性组分。Rp-18反相色谱柱和高效液相色谱仪相结合,从抗菌代谢产物分离纯化出一种抗松梢枯病菌(Sphaeropsis sapinea)的活性物质CXL-I,高效液相色谱检测表明CXL-I为一纯物质。CXL-I对松梢枯病菌的菌丝生长和孢子萌发有较强的抑制活性,当CXL-I浓度为50礸/ml时对菌丝生长抑制率可达80%,对孢子萌发抑制率达95%以上。MS medium was used for the culture of Cyathus gansuensis and inhibitory metabolites against Sphaeropsis sapinea were extracted by CHCl3 and CH3COOCH2CH3 from the ferment liquid of the bird's nest fungus. Leaded by bioassay, a inhibitory compound was obtained by a combination of reversed-phase silica gel column chromatography and HPLC. HPLC analysis indicated that the inhibitory compound was a purified compound and it was named CXL-I. Inhibition effect of CXL-I against the growth of hyphae and germination of spores of Sphaeropsis sapinea was studied. The results indicated that CXL-I could obviously inhibite the growth of hyphae and germination of spores of the forest pathogen. When the dosage of CXL-I was 50g/ml, the relative inhibition rates of hyphae growth and spore germination of the pathogen were 80% and more than 95% respectively.

关 键 词：甘肃黑蛋巢 松梢枯病菌 分离纯化 抑菌作用 

分 类 号：Q939.32[生物学—微生物学]