褐腐真菌产生的羟基自由基HO˙对纤维素作用的研究  

EFFECTS OF HYDROXYL RADICAL HO˙ON CELLULOSE DEGRADATION BY BROWN-ROT FUNGI

作　　者：王蔚[1,2] 段新源[1] 孙彩云[1] 高培基[1] 

机构地区：[1]山东大学微生物技术国家重点实验室 [2]济南大学化学与环境工程学院济南250002

出　　处：《菌物系统》2002年第3期400-405,共6页Mycosystema

基　　金：国家自然科学基金资助项目(39970004)

摘　　要：由褐腐真菌的典型菌株——密粘褶菌Gloeophyllum trabeum的胞外培养液中分离纯化得到一能还原Fe3+,产生羟基自由基HO˙的多肽组分(称作Gt因子)。 采用HO˙特异性的抑制剂硫脲,对Gt因子产生的HO˙在纤维素降解中的作用进行了对照研究,结果表明Gt因子及其产生的HO˙在纤维素降解中起着重要的作用,为褐腐菌HO˙氧化降解纤维素机制假说的确立提供了一些依据。A peptide(named Gt factor) was isolated from the extracellular culture of Gloeophyllum trabeum, the typical brown-rot fungus. Gt factor could reduce Fe3+ to Fe2+ and mediated the production of HO˙ Effects of HO˙on cellulose degradation was comparatively studied with thiourea used as HO˙- specific inhibitor. All results showed that HO˙ produced by Gt factor did have some effects on cellulose degradation, it provided some evidence of HO˙be involved in oxidative mechanism of cellulose degradation by brown-rot fungi.

关 键 词：密粘褶菌 纤维素 HO^·氧化机制 硫脲 

分 类 号：Q939.32[生物学—微生物学]