长期900MHz射频电磁辐射对NIH/3T3细胞凋亡与周期的影响  

Impacts of cell apoptosis and cell cycle of NIH/3T3 cells induced by long-term 900 MHz electromagnetic radiation

作　　者：丁真[1] 李劲涛[1,2] 李凡[1] Vania Helena Melicio Teofilo 周绍梅[1] 吴水才[1] 曾毅[1,3] 

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100124 [2]广西恒煊生物科技有限公司,南宁530007 [3]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《北京生物医学工程》2016年第6期645-650,共6页Beijing Biomedical Engineering

基　　金：国家重点基础研究发展计划(2011CB503700);国家自然科学基金(71661167001);北京市自然科学基金(5162003);北京工业大学校级基础基金(015000514314004)资助

摘　　要：目的探讨长期900 MHz电磁辐射对NIH/3T3细胞凋亡与周期的影响,以为进一步深入研究电磁辐射的细胞生物学效应提供一定理论依据。方法将NIH/3T3细胞分为对照组和辐射组,对照组NIH/3T3细胞为正常条件培养,电磁辐射组用SAR值为4.0 W/kg的900 MHz电磁辐射辐照,辐照时间为2 h/d。分别取正常培养和电磁辐射辐照的4 d、7 d、10 d和12 d的对照组和辐射组NIH/3T3细胞,用流式细胞法进行细胞凋亡和细胞周期检测和分析。结果长期900 MHz电磁辐射引起NIH/3T3细胞出现显著的早期和晚期凋亡。细胞辐照12 d时产生S期阻滞。结论在12 d的辐照周期里,900 MHz电磁辐射持续地诱导NIH/3T3细胞凋亡,在辐照超过10 d后开始对细胞周期产生一定影响。Objective To investigate the biological impacts of cell apoptosis and cell cycle caused by long term 900 MHz electromagnetic radiation（ EMR）,which is crucial for further research of biological impacts induced by electromagnetic radiation. Methods NIH/3T3 cells were divided into Sham and Expo groups. The Expo group was exposed by 900 MHz EMR at an average SAR of 4. 0 W/kg for 2 hours per day,lasting respectively 4,7,10 and 12 days. The Sham group was cultured with no exposure. The cells were collected for cell apoptosis and cell cycle detection by flow cytometry（ FCM） detection. Results The data indicated that 900 MHz EMR apparently induced both early and late apoptosis in NIH/3T3 cells and 900 MHz EMR could block NIH/3T3 cell cycle progression after 12-day exposure. The S phase got arrested after 12-day exposure.Conclusions After exposure to 900 MHz EMR for 12 days at average SAR of 4. 0 W/kg,obvious cell apoptosis was found in the whole progression of exposure. The effects on cell cycle were not clearly found until the cells were exposed to 900 MHz EMR for 10 days.

关 键 词：电磁辐射 细胞凋亡 细胞周期 长期暴露 

分 类 号：R318[医药卫生—生物医学工程]