pH对马精子低温保存效果的影响  

Effect of pH on the Quality of Cooled Stired Equine Sperm

作　　者：毋状元[1] 郑新宝[1] 董红[1] 霍飞[1] 罗永明[1] 于伟浩 徐文慧 陈静波[1] WU Zhuang-yuan ZHENG Xin-bao DONG Hong HUO Fei LUO Yong-ming YU Wei-hao XU Wen-hui CHEN Jing-bo(Institute of Animal Science, Xinjiang Academy of Animal Science, Urumqi 830022, China Zhaosu Bureau of Animal Husbandry and Veterinary, Zhaosu Xinjiang 835600, China)

机构地区：[1]新疆畜牧科学院畜牧研究所,新疆乌鲁木齐830011 [2]昭苏县畜牧兽医局,新疆昭苏835600

出　　处：《家畜生态学报》2016年第12期36-39,共4页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金项目(31360550)

摘　　要：为了研究适宜马精液体外保存pH,本研究选取4匹4~12岁英纯血种公马,收集精液置于pH为6.5、6.7、6.9、7.1、7.3和7.5的INRA82和INRA96稀释液中,低温保存1、24、48和72h后,利用计算机辅助精子分析仪检测评价精子质量。结果表明：INRA96基础稀释液pH为7.1和7.3组保存48h后活精子比例（Total Mobility,TM）分别为81.33%±1.15和80.33%±4.04,显著高于pH为6.5和7.5组的72.67%±3.81和73.00%±4.58（P〈0.05）,与其他各组间无显著差异（P〉0.05）;INRA82基础稀释液pH为6.9组保存48h后活精子比例（Total Mobility,TM）为74.75%±3.51,显著高于pH为6.5和7.5组的67.75%±3.14和52.50%±1.53（P〈0.05）,与其他各组间无显著差异（P〉0.05）。结果提示,马精子体外保存的适宜pH区间为6.9~7.3之间,当pH低于6.7或高于7.3时,随着保存时间的延长精子活力会明显下降。The objective of this study was to investigate the most suitable pH for cooled stired equine semen.4thoroughbred stallions at 4~12years old were selected to collect their semen which were placed in INRA96 or INRA82extender,whose pH was adjusted to 6.5,6.7,6.9,7.1,7.3and 7.5,and then to evaluate the quality of semen after storage 1h,24 h,48hand 72 h.The results show that after stored 48 h the total mobility in INRA96,whose pH were 7.1and 7.3,were 81.33±1.15 and 80.33±4.04 significantly higher than that of pH 6.5（72.67±3.81）and 7.5（73.00±4.58）group（P〈0.05）,with no significant difference with other groups;the total mobility in INRA82,whose pH were 6.9,were 74.75±3.51 significantly higher than pH 6.5（67.75±3.14）and 7.5（52.50±1.53）group（P〈0.05）,with no significant difference with other groups.The data suggest that the most suitable pH is between 6.9~7.3for cooled stired of horse semen.When the pH is lower than 6.7or higher than 7.3,it will be detrimental to the preservation of sperm.

关 键 词：马 精子 低温保存 酸碱度 

分 类 号：S811.5[农业科学—畜牧学]