毛细管区带电泳法测定药物制剂中褪黑素与维生素B_6  

Determination of Melatonin and Pyridoxine in Tablets by Capillary Zone Electrophoresis

作　　者：李金祥[1] 李真[1] 任航[1] LI Jin--xiang LI Zhen REN Hang(School of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian Liaoning 116029,China)

机构地区：[1]辽宁师范大学化学化工学院,辽宁大连116029

出　　处：《德州学院学报》2016年第6期52-55,共4页Journal of Dezhou University

摘　　要：建立一种测定药物制剂中褪黑素和维生素B6的毛细管区带电泳-紫外检测方法.分离条件优化后,对该方法进行了评价.结果表明,褪黑素与维生素B6分别在3.6~50 mg/L和2.7~50 mg/L内线性良好（R2=0.9972,0.9893）,加标回收率分别为87.6%~104.9%和92.1%~102.7%,精密度分别为1.65%和1.01%（RSD%,n=6）,检出限分别为1.5mg/L和1.0mg/L,可满足药物制剂中褪黑素与维生素B6含量测定要求.此外,该方法还具有简单易行,检测周期短,分析成本低等优点.A method for determination of melatonin and pyridoxine was proposed by capillary zone electro- phoresis. After optimization, the method was evaluated. The linear ranges were from 3.6 to 50 mg/L （R2 =0. 9972） for melatonin and from 2.7 to 50 mg/L （R2=0. 9893） for pyridoxine. The recoveries （n=3）, precisions （RSD%,n=3） ,detection limits were 87. 6%--104. 9M and 92. 1%--102. 7%,1.65% and 1.01 % , 1.5 mg/L and 1.0 mg/L for melatonin and pyridoxine,respectively. This method was finally ap- plied to the,determination of melatonin and pyridoxine in eommercial tablets, and satisfactory results were obtained. In addition, the proposed method has the merits of easy operation, high throughout and low cost, suggesting a good potential to be used for the quality control and inspection in the drug production and circulation.

关 键 词：毛细管区带电泳 褪黑素 维生素B6 

分 类 号：O657.7[理学—分析化学]