去细胞化全肝生物支架修复肝损伤  

Decellularized whole liver bioscaffold repairs liver injury

作　　者：张庆峰 李自荣[2] 

机构地区：[1]山东医学高等专科学校附属医院,山东省临沂市276000 [2]青岛大学医学院,山东省青岛市266021

出　　处：《中国组织工程研究》2016年第52期7809-7814,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:去细胞化支架是一类特殊的支架,它保留了组织中的管道结构,解决了细胞生长营养输送问题,并且能为细胞提供近似体内的生长环境,具有其他修复材料无法比拟的优势。目的:研究去细胞化全肝生物支架的制备方法,探讨去细胞化全肝生物支架修复肝损伤的效果。方法:取SD大鼠12只,采用化学去垢剂-酶联合去细胞化技术制备去细胞化全肝生物支架。另取SD大鼠24只,建立肝损伤模型,随机分为2组,实验组肝损伤部位置入去细胞化全肝生物支架,对照组于肝损伤部位注射生理盐水。术后30 d,检测血清丙氨酸转氨酶及谷草转氨酶水平,取肝脏组织进行苏木精-伊红染色观察。结果与结论:(1)去细胞化全肝生物支架的结构:苏木精-伊红染色可见细胞外基质样结构存在;电镜下可见肝脏内细胞成分被完全去除,支架胶原纤维排列整齐,未出现溶解现象;(2)血清丙氨酸转氨酶及谷草转氨酶水平:实验组丙氨酸转氨酶及谷草转氨酶水平显著低于对照组(P<0.05);(3)肝脏组织苏木精-伊红染色:对照组可见大量蓝染并密集分布的细胞核及粉红色分布结构,纤维胶原之间并未出现明显断裂;实验组可见粉红色且分布密集结构;(4)结果表明:采用化学去垢剂-酶联合去细胞化技术制备去细胞化全肝生物支架方法相对简单,去细胞化全肝生物支架可促进肝损伤的修复。BACKGROUND：Decel ularized scaffolds are special for retaining the tubular structure used for nutrition transport, and providing a similar inner environment for cel growth. OBJECTIVE：To study the preparation of the decel ularized whole liver bioscaffold and to explore its repair outcomes for liver injury. METHODS：Livers from 12 Sprague-Dawley rats were used for preparing the decel ularized whole liver bioscaffold by chemical detergent-enzymes decel ularized technology. Models of liver injury were established in another 24 Sprague-Dawley rats and randomized into two groups：the decel ularized whole liver bioscaffold was implanted into the rat liver lesions in experimental group, and controls were given the injection of normal saline. Thirty days later, the serum levels of alanine aminotransferase and glutamic-oxaloacetic transaminase were detected, and liver tissues were removed for hematoxylin-eosin staining. RESULTS AND CONCLUSION：Hematoxylin-eosin staining showed that extracel ular matrix-like structures existed in the decel ularized bioscaffold;cel components were completely removed from the liver, the col agen fibers in the scaffold arranged regularly and were not dissolved under electron microscope. The serum levels of alanine aminotransferase and glutamic-oxaloacetic transaminase in the experimental group were significantly lower than those in the control group （P〈0.05）. Hematoxylin-eosin staining showed a large number of blue-stained and dense distributed nuclei, and pink distribution of col agen fibers that had no overt breakages in the control group, while pink and dense structures in the experimental group. These results suggest that the decel ularized whole liver bioscaffold is easy to obtain, and can promote the injured liver repair.

关 键 词：肝 人工 转氨酶类 组织工程 生物材料 材料相容性 去细胞化全肝生物支架 制备方法 肝损伤 修复效果 化学去垢剂 转氨酶 

分 类 号：R318[医药卫生—生物医学工程]