复合左旋聚乳酸胆肠溶解支架的体外实验  

In vitro study on a degradable poly-L-lactic acid biliary-enteric composite stent

作　　者：姚金锋[1] 韩金丽[2] 郭昱[1] 崔东来[1] 

机构地区：[1]河北医科大学第二医院消化内科,河北省石家庄市050000 [2]保定第七医院内镜室,河北省保定市072150

出　　处：《中国组织工程研究》2016年第52期7815-7820,共6页Chinese Journal of Tissue Engineering Research

基　　金：2012年度医学科学研究重点课题(201200817)~~

摘　　要：背景:目前已研制出复合左旋聚乳酸可降解性胆肠支架。目的:研究复合左旋聚乳酸胆肠溶解支架的溶解性及生物相容性。方法:(1)溶解性:将复合左旋聚乳酸胆肠溶解支架置于人工胃酸中,12周内检测溶解率;(2)热源性:将复合左旋聚乳酸胆肠溶解支架浸提液经耳缘静脉注入兔体内,检测体温变化;(3)溶血性:将复合左旋聚乳酸胆肠溶解支架浸提液、生理盐水及蒸馏水分别加入兔抗凝血中,检测溶血率;(4)细胞相容性:分别以含体积分数10%胎牛血清的DMEM高汤培养基、橡胶材料浸提液、复合左旋聚乳酸胆肠溶解支架浸提液培养结肠癌Caco-2细胞,12,24,36,48 h后,检测细胞增殖;2 d后,检测细胞乳酸脱氢酶释放量。结果与结论:复合左旋聚乳酸胆肠支架的体内稳定性时间长,在20周左右可以溶解近80%以上;复合左旋聚乳酸胆肠支架不具有热源性及溶血性;复合左旋聚乳酸胆肠支架对Caco-2细胞的增殖及乳酸脱氢酶释放无影响,无明显细胞毒性;结果表明,复合左旋聚乳酸材料具有良好的溶解性及细胞相容性。BACKGROUND：The degradable poly-L-lactic acid （PLLA） biliary-enteric composite stent has been developed. OBJECTIVE：To analyze the solubility and biocompatibility of the degradable PLLA biliary-enteric composite stent. METHODS：Solubility：the PLLA composite stent was implanted into artificial gastric acid to detect the dissolution rate within 12 weeks. Pyrogen test：the PLLA composite stent extracts were injected into the rabbits via ear vein to detect the changes of body temperature. Hemolysis test：the PLLA composite stent extracts, normal saline and distil ed water were added into the rabbit anticoagulant, respectively, to detect the hemolysis ratio. Cytocompatibility test：Caco-2 cel s were respectively cultured in the DMEM medium containing 10%fetal bovine serum, rubber material extracts and the PLLA composite stent extracts, and the cel proliferation was detected at 12, 24, 36 and 48 hours;the lactic dehydrogenase release was detected at 2 days. RESULTS AND CONCLUSION：The PLLA composite stent showed a long stability in vivo, and approximately 80%was dissolved at about 20 weeks. No reactions of pyrogen and henolysis were observed in the pyrogen and hemolysis tests. The PLLA composite stent made no effects on the Caco-2 cel proliferation and lactic dehydrogenase release. In conclusion, the PLLA composite material holds a good solubility and cytocompatibility.

关 键 词：生物相容性材料 乳酸脱氢酶类 组织工程 生物材料 材料相容性 左旋聚乳酸 聚乳酸乙醇酸 溶解率 热源实验 溶血实验 CCK-8 Caco-2细胞 乳酸脱氢酶 

分 类 号：R318[医药卫生—生物医学工程]