磷酸钙骨水泥/纤维蛋白胶复合材料填充桡骨缺损的生物相容性  

Biocompatibility of calcium phosphate cement/fibrin glue in filling radius defects

作　　者：黄田[1] 郑南生[2] 张育专 吴永乐[1] 王刚[1] 

机构地区：[1]海南省第二人民医院,海南省五指山市572299 [2]海南省中医院,海南省海口市570203

出　　处：《中国组织工程研究》2016年第52期7829-7835,共7页Chinese Journal of Tissue Engineering Research

摘　　要：背景:磷酸钙骨水泥与人体自身骨矿物的化学组成和结构相似,能够填充因骨折造成的骨质塌陷,具有诱导成骨作用,但其降解速度较慢。目的:评估磷酸钙骨水泥/纤维蛋白胶复合材料的生物相容性及修复桡骨缺损的可行性。方法:(1)体外细胞毒性实验:分别以磷酸钙骨水泥/纤维蛋白胶材料浸提液、苯酚溶液,含体积分数为10%胎牛血清RPMI1640培养液培养小鼠成纤维细胞,检测细胞毒性分级;(2)血液相容性实验:在兔抗凝血中分别加入磷酸钙骨水泥/纤维蛋白胶材料浸提液、生理盐水及双蒸水,检测溶血率;(3)取45只新西兰大白兔,制备双侧桡骨缺损模型,随机分3组,空白对照组不进行任何干预,实验组与骨缺损处植入磷酸钙骨水泥/纤维蛋白胶复合材料,对照组植入自体桡骨。术后4,8,16周,进行X射线、组织学、骨密度及生物力学检测。结果与结论:(1)细胞毒性:磷酸钙骨水泥/纤维蛋白胶材料浸提液的毒性为0至1级;(2)血液相容性:磷酸钙骨水泥/纤维蛋白胶材料的溶血率为3.15%;(3)体内修复实验:术后16周,X射线显示,实验组和对照组骨折线完全消失,髓腔复通,塑性完全;组织学显示,对照组骨小梁重建较明显,板层骨较成熟,髓腔再通,实验组有大量新生编织骨成网格状长入材料中,材料降解明显,降解与骨长入同步;实验组与对照组骨密度、最大负荷、最大应力和破坏能量均明显高于空白对照组(P<0.05),实验组与对照组各指标比较差异无显著性意义;(4)结果表明:磷酸钙骨水泥/纤维蛋白胶复合材料具有良好的生物相容性,修复骨缺损可促进骨组织再生,获得与自体骨移植基本相当的治疗效果。BACKGROUND：The chemical compositions and structure of calcium phosphate bone cement are similar to those of human bone, which can fil the bone colapse caused by fracture and induce osteogenesis, but its degradation rate is slow. OBJECTIVE：To evaluate the biocompatibility of the calcium phosphate cement/fibrin glue and the feasibility of repairing radius defects. METHODS： In vitro cytotoxicity experiment： Mouse fibroblasts were cultured in the calcium phosphate bone cement/fibrin glue extracts, phenol solution, and RPMI 1640 culture medium containing 10% fetal bovine serum, respectively, to detect the cytotoxicity grade. Hemocompatibility experiment： Calcium phosphate bone cement/fibrin glue extracts, normal saline and distiled water were respectively added into the rabbit anticoagulation, to detect the hemolytic rate. Forty-five New Zealand white rabbits were enroled and modeled into bilateral radius defects, folowed by randomly alotted into three groups： blank control group without any intervention, experimental and control groups were given the implantation with calcium phosphate bone cement/fibrin glue and autologous radius, respectively. X-ray, histology, bone mineral density and biomechanical test were performed at postoperative 4, 8 and 16 weeks. RESULTS AND CONCLUSION：The toxicity grade of the calcium phosphate cement/fibrin glue was 0 to 1. The hemolytic rate of the calcium phosphate cement/fibrin glue was 3.15%. At 16 weeks postoperatively, X-ray showed that in the experimental and control groups, the fracture line disappeared completely, pulp cavity was recanalized, and in plastic completely. Histology showed that the reconstructed bone trabecular was obvious, plate layer of bone was mature, and medulary cavity recanalization appeared in the control group; there were a large number of new grid-shaped woven bone tissues growing into the material in the experimental group, with overt degradation, and degradation rate was in paralel to bone ingrowth. The bone density, the maximum load, maximu

关 键 词：磷酸钙类 纤维蛋白组织粘着剂 组织工程 生物材料 骨生物材料 桡骨缺损 磷酸钙骨水泥/纤维蛋白胶 生物相容性 动物模型 

分 类 号：R318[医药卫生—生物医学工程]