清开灵、双黄连联合头孢哌酮-舒巴坦钠对产ESBLs肺炎克雷伯菌抗菌作用研究  

Antibacterial effect of Qingkailing injection,Shuanghuanglian powder-injection combined with cefoperazone-sulbactam sodium on ESBLs-producing Klebsiella pneumonia

作　　者：梁华[1] 彭小宝 杨军平[1] 杨建安[1] 周爱明[3] 

机构地区：[1]江西中医药大学附属医院检验科,江西南昌330006 [2]江西新余市中医院,江西新余336500 [3]江西中医药大学附属医院药剂科,江西南昌330006

出　　处：《实验与检验医学》2016年第6期723-727,共5页Experimental and Laboratory Medicine

基　　金：江西省卫生计生委中医药科研课题(2014A060)

摘　　要：目的探讨中药清开灵、双黄连对产ESBLs肺炎克雷伯菌的抗菌活性。方法取分离自临床标本的产ESBLs肺炎克雷伯菌,建立耐药菌模型,并将实验菌液以5×10~5cfu/ml接种量预加到试管中;配制清开灵、双黄连、头孢哌酮-舒巴坦钠药液,按药物不同组合和药物作用时间的不同进行实验分组,并采用对倍稀释法分别加入到含有实验菌液的相应试管中,35℃培养24h,肉眼观察抑制细菌生长的最低药物浓度即最低抑菌浓度（MIC）。结果清开灵、双黄连、头孢哌酮-舒巴坦钠对产ESBLs肺炎克雷伯菌的MIC分别是31.25ml/L、7.5g/L、117mg/L,清开灵联合应用头孢哌酮-舒巴坦钠至最佳效应组合时,二者MIC分别降低了93.76%和75%;双黄连联合应用头孢哌酮-舒巴坦钠至最佳效应组合时,二者MIC分别降低了75%和87.52%。结论清开灵注射剂和双黄连粉针对产ESBLs肺炎克雷伯菌具有抑制作用,两药分别与头孢哌酮-舒巴坦钠联合应用具有明显的协同作用,可以有效提高抗生素对耐药菌的敏感性,尤其以中药作用6h为最佳。Objective To investigate the antibacterial activity of Chinese herbal in extended spectium β-lactamases（ESBLs）-producing Klebsiella pneumonia.Methods ESBLs positive Klebsiella pneumonia were collected from clinic samples for establishing the drug-resistance model.The sterile test tubes were divided into different test groups according to different Chinese herbals and impact time and the inoculation bacteria liquid was 5×10~5cfu/ml.The medical liquid of Qingkailing injection,Shuanghuanglian powder-injection and cefoperazone-sulbactam sodium were prepared and added into tubes with test bacteria liquid by two-foll diluted.After cultivated at 35℃ for 24 hours,the lowest concentration would be minimum inhibitory concentration（MIC） by macroscopic observation.Results The MIC of Qingkailing injection,Shuanghuanglian powder-injection and cefoperazone-sulbactam sodium were respectively 31.25ml/L,7.5g/L and 117mg/L.The MIC of Qingkailing and cefoperazone-sulbactam sodium respectively dropped by 93.76% and 75% when Qingkailing was combined with cefoperazone-sulbactam sodium;when Shuanghuanglian was combined with cefoperazone-sulbactam sodium,the MIC of Shuanghuanglian dropped by 75% and that of cefoperazone-sulbactam sodium dropped by 87.52%.Conclusion The test proves that Qingkailing injection and Shuanghuanglian powder-injection have inhibitory effect on ESBLs-producing Klebsiella pneumonia.When they are combined with cefoperazone-sulbactam sodium,they will have significant synergisms respectively and the sensitivity of antibiotics to drug-resistance bacteria can be improved,especially after Chinese herbal played a role for 6 hours.

关 键 词：清开灵 双黄连 耐药肺炎克雷伯菌 抑菌作用 

分 类 号：R446.5[医药卫生—诊断学] Q939.92[医药卫生—临床医学]