通体沉香对小鼠催眠和自主活动抑制作用  

Effect of agarwood produced by whole-tree agarwood-inducing technique on hypnotic and spontaneous activity inhibition of mice

作　　者：王帅[1] 周岳[1] 马富超 张泉洋 刘洋洋[3] 弓宝[3] 郭鹏[1,3] 魏建和[1,3] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]哈尔滨商业大学生命科学与环境科学研究中心,哈尔滨150076 [3]中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室,万宁571533

出　　处：《国际药学研究杂志》2016年第6期1082-1087,共6页Journal of International Pharmaceutical Research

基　　金：国家自然科学基金资助项目(81173481);海南省重大科技计划资助项目(ZDXM20120033);海南省中药现代化专项资助项目(2015ZY09)

摘　　要：目的基于"通体结香技术"所产沉香,评价其不同部位在镇静催眠方面的药效学作用,为今后临床应用及产品开发提供科学依据。方法针对通体沉香的不同提取部位(水提物、醇提物及挥发油),通过协同戊巴比妥钠阈上和阈下催眠实验,观察小鼠入睡率、入睡潜伏期和睡眠时间;通过自主活动实验,观察小鼠运动路程、运动时间、平均速度等指标,评价通体沉香在镇静催眠方面的药理功效,并与市售野生沉香进行比较。结果协同戊巴比妥钠催眠实验结果显示,与空白对照相比,通体沉香醇提物和挥发油均能显著增加入睡率和延长睡眠时间(P<0.05),挥发油还能显著缩短入睡潜伏期(P<0.05);自主活动实验表明,与空白对照相比,醇提物和挥发油均能够显著减少总路程、运动路程和运动时间,降低平均速度(P<0.05);而水提物作用不明显(P>0.05)。结论利用"通体结香技术"所产沉香具有镇静催眠作用,其作用与市售沉香一致甚至优于市售沉香。Objective To evaluate the sedative-hypnotic effect of agarwood different extracts produced by whole-tree agarwood-inducing technique（Agar-Wit agarwood）to make scientific basis for the Agar-Wit agarwood exploration and usage in clinic.Method Collaborative pentobarbital sodium hypnosis experiment was employed to assess the index of fell asleep rate,sleep latency time and sleep time. And locomotor activity assay was used to test the index of moved distance,travel time and average velocity. Then a functional comparison was made between Agar-Wit agarwood and commereial agarwood. Result The Agar-Wit agarwood ethanol extract and essential oil could significantly increase sleep rate and prolong sleep time. At the same time,essential oil could also decrease sleep latency time significantly. Additionally,both ethanol extract and oil significantly decreased the mice locomotor activity,including reduced total distance,movement,move time and average velocity. However,the water extract did not have significant effect.Conclusion The Agar-Wit agarwood ethanol extract and oil have significant effect on sedation in mice,whose function is similar to,or even better than that of the commerical agarwood.

关 键 词：通体沉香 植物提取物 挥发油：催眠 自主活动 

分 类 号：R285.5[医药卫生—中药学]