丹参酮ⅡA对成骨细胞增殖的影响  

Effect of Tanshinone ⅡA on the Proliferation of Osteoblasts

作　　者：叶茂[1] 郑勇[1] 刘艳西[1] 陈明[1] 胡锋[1] 

机构地区：[1]湖北科技学院附属第一医院脊柱关节外科,湖北咸宁437100

出　　处：《湖北科技学院学报（医学版）》2016年第6期461-463,共3页Journal of Hubei University of Science and Technology(Medical Sciences)

摘　　要：目的探讨丹参酮ⅡA对成骨细胞增殖的影响,为丹参酮ⅡA治疗骨质疏松提供实验依据。方法通过体外培养成骨细胞,实验分为四组:对照组、丹参酮ⅡA低剂量组(5μM)、丹参酮ⅡA中剂量组(10μM)、丹参酮ⅡA高剂量组(20μM)。采用分光光度计测量OD值检测成骨细胞的增殖活性;采用二乙醇胺法测定细胞内碱性磷酸酶活性;使用流式细胞仪检测成骨细胞的凋亡率。结果丹参酮ⅡA中、高剂量组与对照组相比OD值明显增加(P<0.05、P<0.01),提示其可提高成骨细胞的增殖活性。与对照组相比,丹参酮ⅡA中、高剂量组细胞内碱性磷酸酶活性明显升高(P<0.05),而凋亡率则明显下降(P<0.05)。结论参酮ⅡA能促进成骨细胞增殖,其机制可能是通过抑制成骨细胞的凋亡,为其临床上用于骨质疏松的治疗提供实验依据。Objective To investigate the effect of Tanshinone ⅡA on the proliferation of osteoblasts.Methods The osteoblasts were cultured in vitro and divided into four groups： control group,low dose of Tanshinone ⅡA group（ 5 μM）,middle dose of Tanshinone Ⅱ A group（ 10 μM） and high dose of Tanshinone Ⅱ A group（ 20 μM）. The proliferation activity of osteoblasts was detected with CCK-8 kit. Intracellular alkaline phosphatase activity was determined by two ethanol amine method. Apoptosis rate of osteoblasts was detected by flow cytometry. Results The proliferation activity was significantly increased in middle and high doses of Tanshinone II A groups and there was statistical significance as compared to the control group（ P〈0. 05 or P〈0. 01）.Compared with the control group,the activity of alkaline phosphatase activity in high dose group was increased（ P〈0. 05）,while the apoptosis rate was decreased（ P〈0. 05）. Conclusion Tanshinone II A can promote the proliferation of osteoblasts partly by inhibiting the apoptosis of osteoblasts,which may provide experimental evidence for the treatment of osteoporosis.

关 键 词：丹参酮ⅡA 成骨细胞 增殖 凋亡 

分 类 号：R965.2[医药卫生—药理学]