烟曲霉荧光探针实时PCR法的临床应用价值研究  

Preliminary exploration on clinical application value of aspergillus fumigatus fluorescence probe real-time PCR method

作　　者：邢志芳[1] 曹国君[2] 赵缜[3] 赵红英[4] 潘惠芬[3] 夏建红[3] 

机构地区：[1]复旦大学附属闵行医院输血科,上海201199 [2]复旦大学附属华山医院检验医学科,上海200040 [3]复旦大学附属闵行医院检验科,上海201199 [4]潍坊医学院,山东潍坊261053

出　　处：《国际检验医学杂志》2016年第24期3391-3392,共2页International Journal of Laboratory Medicine

基　　金：上海市卫生和计划生育委员会青年项目(20154Y0141);上海市闵行区科学技术委员会自然基金项目(2015MHZ003;2016MHZ01);上海市闵行区卫生和计划生育委员会基金项目(2014MW13)

摘　　要：目的建立一种针对烟曲霉的探针实时聚合酶链反应(PCR)法并初步探讨其临床应用价值。方法以烟曲霉基因组内特有序列为靶位,设计引物、探针,建立PCR反应体系,初步应用于临床痰标本和血浆标本的检测验证其检测性能。结果所建针对烟曲霉的实时PCR法具有良好的灵敏度和特异度,将其应用于临床痰标本和血浆标本的检测效果较好。结论所建立的荧光探针实时PCR法初步应用于临床痰标本和血浆标本检测,效果良好,其实际临床应用价值仍需进行大样本验证。Objective To establish a probe real-time polymerase chain reaction（PCR）method for aspergillus fumigatus and to preliminarily explore its clinical application value.Methods The special genome sequence of aspergillus fumigatus served as the target for designing corresponding primers and probe and establishing the PCR reaction system.This method was preliminarily applied in the detection of clinical sputum and plasma samples for verifying its detection performance.Results The established real time PCR method for aspergillus fumigatus had good sensitivity and specificity.The method showed good effect in detecting the clinical sputum and plasma samples.Conclusion The established fluorescence probe real-time PCR method for aspergillus fumigatus has good effect in preliminary application of clinical sputum and plasma sample detection.But its practical clinical value still needs to be verified by a larger sample.

关 键 词：烟曲霉菌 探针实时聚合酶链反应法 临床应用 

分 类 号：R519[医药卫生—内科学] R440[医药卫生—临床医学]