不同乳汁成分乙型肝炎病毒DNA定量检测的结果分析  

Analysis of hepatitis B virus DNA quantitative detection results in different breast milk compositions

作　　者：汪东剑[1] 余维涛[1] 刘冬萍[1] 张晓云[1] 邱华琴[1] 刘仙[1] 

机构地区：[1]中国人民解放军第一八四医院输血科,江西鹰潭335000

出　　处：《国际检验医学杂志》2016年第24期3403-3405,共3页International Journal of Laboratory Medicine

基　　金：南京军区医学科技创新课题资助项目(MS078)

摘　　要：目的分析不同乳汁成分中乙型肝炎病毒(HBV)-DNA定量检测的结果,为乳汁标本HBV-DNA检测提供参考和依据。方法对152例乙肝产妇乳汁分别选用混匀乳汁、乳酪、乳清和沉淀进行HBV-DNA定量检测。结果选用不同的检测对象时,HBV-DNA检测的阳性率差异无统计学意义(χ2=0.437,P=0.933);HBV-DNA载量间差异有统计学意义(F=2.835,P=0.043),沉淀中HBV-DNA载量高于乳清中HBV-DNA载量;两两比较后,混匀乳汁、乳酪、乳清间检测结果差异无统计学意义(P>0.05),混匀乳汁、乳酪、沉淀间检测结果差异无统计学意义(P>0.05)。结论对乳汁标本进行HBV-DNA定量检测时,混匀乳汁可能是更为适合的检测对象。Objective To analyze the results of hepatitis B virus（HBV）-DNA quantitative detection in different breast milk components to provide reference and basis for the HBV-DNA quantitative detection of breast milk sample.Methods The HBV-DNA in the blending milk,cheese,whey and precipitation in 152 hepatitis B parturients was quantitatively detected.Results When choosing different detect objects,there was no statistically significant difference in HBV-DNA detection positive rate（χ^2=0.437,P=0.933）;the difference in HBV-DNA loads was statistically significant（F=2.835,P=2.835）,the HBV-DNA loads in precipitation was higher than that in whey.There was no statistical significant difference in the pairwise comparison of the test results of blending milk,cheese and whey;also the difference of test results among blending milk,cheese and precipitation was not statistically significant（P〉0.05）.Conclusion In the HBV-DNA quantitative detection of breast milk sample,the blending milk may be more suitable detection object.

关 键 词：乳汁 乙型肝炎病毒 DNA 

分 类 号：R512.62[医药卫生—内科学]