不同冷冻保护剂对羔羊睾丸组织冷冻效果的影响  

Effect of Different Cryoprotectants on Activity of Cryopreserved Lamb Testicular Tissue

作　　者：朱庆超[1] 郭奇奇 王艳华[1,2] 姜雪梅[1] 方乾[1] 李浩[1] 胡建宏[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]拉萨市动物疫病预防控制中心,拉萨850000

出　　处：《西北农业学报》2016年第12期1762-1767,共6页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家绒毛用羊产业技术体系(CARS-40-13);陕西省科技统筹创新工程计划(2015KTTSNY04-03)~~

摘　　要：为探讨非渗透性冷冻保护剂与渗透性冷冻保护剂配伍对羔羊睾丸组织冷冻效果的影响,以φ=10%DMSO、φ=10%EG、φ=10%DMSO+5g/L BSA和φ=10%EG+5g/L BSA为冷冻保护剂,测定冷冻前后羔羊睾丸组织的细胞活率、曲细精管完整率、睾酮浓度、抑制素质量浓度以及碱性磷酸酶(AKP)和酸性磷酸酶(ACP)活性。结果表明,φ=10%DMSO+5g/L BSA组的细胞活率、曲细精管完整率、睾酮浓度、抑制素的质量浓度及AKP和ACP活性均显著高于其他处理组,但显著低于对照组(新鲜组织);φ=10%EG+5g/L BSA组与φ=10%DMSO组的AKP和ACP活性差异不显著,但均显著高于φ=10%EG组。说明φ=10%DMSO中添加5g/L的BSA能有效改善羔羊睾丸组织的冷冻效果。In order to research the effects of non-permeating cryoprotectants and permeating cryoprotectants combination on lamb testicular tissue cryopreservation,four cryoprotectants（φ=10% DMSO,φ=10% EG,φ=10% DMSO＋5g/L BSA andφ=10% EG＋5g/L BSA）were used in this experiment.The cell viability,integrity of seminiferous tubule,concentration of testosterone,mass concentration of inhibin,activity of alkaline phosphatase（AKP）and acid phosphatase（ACP）were detected both before and after the cryopreservation of lamb testicular tissue.The result showed that the cell viability,rate of seminiferous tubule integrity,concentration of testosterone,mass concentration of inhibin,activity of AKP and ACP inφ=10% DMSO＋5g/L BSA group were significantly higher than in other frozen-thawed groups,while significantly lower than in control group（fresh tissue）.Inφ=10% DMSO group andφ=10% EG＋5g/L BSA group,all the parameters were significantly higher than inφ=10% EG group,and there was no significant difference between these two groups.In conclusion,combining 5g/L BSA andφ=10% DMSO could improve the cryoprotective effects of lamb testicular tissue.

关 键 词：羔羊 睾丸组织 冷冻保存 冷冻保护剂 

分 类 号：S826[农业科学—畜牧学]