严重烧伤后早期大鼠血糖测量方法探讨  

Methods of blood glucose measurement for early-stage severely burned rats

作　　者：左璐 李伟人[2] 鲁加祥[2] 喻翔[2] Zuo Lu Li Weiren Lu Jiaxiang Yu Xiang(Medical Cosmetology Department, the Second People＇s Hospital of Guizhou Province, Guiyang 550000, China Burn and Plastic Surgery Department, the Affiliated Hospital of Guiyang Medical college, Guiyang 550004, China)

机构地区：[1]贵州省第二人民医院医疗美容科,贵阳550000 [2]贵阳医学院附属医院烧伤整形外科,贵阳550004

出　　处：《中华内分泌外科杂志》2016年第6期483-486,共4页Chinese Journal of Endocrine Surgery

基　　金：贵州省卫生厅科学技术基金（gzwkj2011-1-022）

摘　　要：目的分别采用4种方法测定严重烧伤后早期大鼠血糖并比较采用不同检测仪器和标本类型测定血糖结果的差异。方法将24只大鼠按随机数字表法分为对照组A1（8只）与烫伤组B1（16只），烫伤组B1于伤后12、24h采血（每个时相8只）。另外取20只大鼠按随机数字表法分为对照组A2（10只）与烫伤组B2（10只），烫伤组B2于伤后12h采血。烫伤组B1、B2大鼠置于（95．0±0．5）℃热水中15s，制作30％总体表面积（total body surface area，TBSA）、IU度烫伤模型。烫伤组B1（即时复苏）伤后立即腹腔内注射生理盐水（40ml／kg），烫伤组B2（延迟复苏）伤后6h腹腔内注射生理盐水（40ml／kg）。对照组A1、A2仅37℃温水中15s模拟烫伤，不进行液体复苏。用血糖仪／尾动（静）脉全血法、血糖仪／腹主动脉全血法、分光光度计，股静脉血浆法、分光光度计，腹主动脉血浆法4种方法测定大鼠血糖。结果①与对照组A1比较，烫伤组B1伤后12、24h血糖仪／尾动（静）脉全血血糖、分光光度计／股静脉血浆血糖均升高，差异均有统计学意义（均P〈0．05）；与对照组A2比较，烫伤组B2伤后12h血糖仪，腹主动脉全血血糖、分光光度计／腹主动脉血浆血糖均升高，差异均有统计学意义（均P〈0．05）。②对照组A1的血糖仪／尾动（静）脉全血血糖与分光光度计／股静脉血浆血糖比较、对照组A2的血糖仪／腹主动脉全血血糖与分光光度计／腹主动脉血浆血糖比较差异均无统计学意义（P〉．05）；烫伤组B1的血糖仪／尾动（静）脉全血血糖显著低于分光光度计／殷静脉血浆血糖（P〈0．05）；烫伤组B2的血糖仪，腹主动脉全血血糖与分光光度计／腹主动脉血浆血糖比较差异无统计学意义（P〉0．05）。结论在大鼠严重烧伤后早期，4种血糖测量方法均表明血糖升高，用腹主动脉全血采用血糖仪�Objective To compare the differences of blood glucose detected by four methods with differ- ent instruments and specimen types at early stage in severely burned rats. Methods 24 Sprague-Dawley （SD） rats were randomly divided into two groups： control group 1 （Sham scald group, n=8） and scald injury group 1 （n=16）. Blood samples of scald injury group 1 were collected at 12, and 24 hours after scald （n=8, each time）. Another 20 SD rats were randomly divided into two groups： control group 2 （Sham scald group, n=10） and scald injury group 2 （n=10）. Blood samples of scald injury group 2 were collected at 12 hours after scald. The rats in scald injury group 1 and 2 were placed into scalding water （95.0±0.5）℃ for 15 seconds to model third-degree burn with 30% total burn surface area （TBSA）. The rats in scald injury group 1 were given intraperitoneal injection with normal saline（40 ml/kg） immediately, while those in scald injury group 2 were given intraperitoneal injection with normal saline （40 ml/kg） 6 hours after scald. The rats in Sham scald group 1 and 2 were placed into warm water 37℃ for 15 seconds, and did not received injection. Portable glucometer/caudal artery （vein） blood, portable glueometer/abdominal aorta blood, spectrophotometer/femoral venous plasma, and spectrophotometer/abdominal aorta plasma were used to detect blood glucose. Results （1）Compared with Sham scald group 1, the levels of blood glucose detected by portable glucometer/caudal artery （vein） blood and spectrophotometer/femoral venous plasma in scald injury groupl at 12, 24 hours after scald were significantly increased（P〈0.05）. Compared with Sham scald group 2, the levels of blood glucose detected by portable glucometer/abdominal aorta blood and spectrophotometer/abdominal aorta plasma in scald injury group 2 at 12 hours after scald were significantly increased（P〈0.05）. （2）The comparison of portable glucometer/caudal artery （vein） blood and spectrophotometer/femora

关 键 词：烧伤 大鼠 血糖 

分 类 号：R644[医药卫生—外科学]