青岛地区儿童淋巴瘤中EB病毒潜伏膜蛋白1基因变异及意义  

Gen mutations of Epstein-Barr virus-encoded latent membrane protein 1 in pediatric lymphomas of Gingdao area

作　　者：岳康[1,2] 高翔翔 李斐斐[1] 王云[1] 

机构地区：[1]青岛大学医学院,山东青岛266021 [2]青岛大学附属医院 [3]青岛市城阳区流亭卫生院

出　　处：《山东医药》2016年第45期19-22,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81171571);青岛市科技计划项目(13-1-3-50-nsh)

摘　　要：目的了解青岛地区儿童淋巴瘤中EB病毒(EBV)潜伏膜蛋白1(LMP1)基因多态性。并探讨LMP1变异在儿童淋巴瘤发生中的意义。方法采用巢式PCR和DNA测序检测66例青岛地区EBV阳性儿童淋巴瘤组织中LMP1全长序列,根据其特征性突变和系统进化树,对基因变异进行分类,并与以往同一地区成人NK/T细胞淋巴瘤和健康人群中LMP1变异类型的分布进行比较。结果 66例标本完成测序,共发现3种LMP1亚型,即China 1、China 2和Med-,其构成比分别为84.8%、7.6%和6.1%,另有1例(1.5%)为China 1/China 2重组病毒株。LMP1 N端XhoⅠ位点缺失变异即XhoⅠ(-)和未缺失型XhoⅠ(+)的检出率分别为93.9%和6.1%;C端30bp缺失即del-LMP1和未缺失型wt-LMP1的检出率分别为84.8%和15.2%。儿童淋巴瘤和成人NK/T细胞淋巴瘤中LMP1各亚型的分布差异无统计学意义,但儿童淋巴瘤中China 1亚型、XhoⅠ(-)和del-LMP1的检出率高于健康人群(P均<0.05)。结论青岛地区儿童淋巴瘤中LMP1以China 1亚型、XhoⅠ(-)和del-LMP1为主,LMP1变异可能与儿童淋巴瘤的发生相关。Objective To understand the gene polymorphisms of Epstein-Barr virus（ EBV）-encoded latent membrane protein 1（ LMP1） gene in pediatric patients with lymphoma from Qingdao area and to investigate the significance of LMP1 in the development of pediatric lymphomas. Methods Nested-PCR and DNA sequencing were used to analyze the gene sequences of LMP1 in isolates from 66 cases of EBV-positive samples of children with lymphomas. The sequences of LMP1 were classified according to the signature changes and the phylogenetic tree. The distribution of LMP1 variants in pediatric lymphoma cases was compared with the previous data from adult NK/T cell lymphoma cases and healthy donors in the same area. Results Three subtypes of LMP1 gene,China 1,China 2 and Med-,were observed in 66 isolates,with distribution of 84. 8%,7. 6% and 6. 1%,respectively. Another 1（ 1. 5%） isolate was recombinant strain of China 1 and China 2.The frequencies of Xho I（-） variant（ loss Xho I site in N-terminus of LMP1） and prototype Xho I（ ＋） were 93. 9% and6. 1%,respectively,and those of del-LMP1 variant（ 30 bp deletion in C-terminus of LMP1） and prototype wt-LMP1 were84. 8% and 15. 2%,respectively. The distribution of LMP1 variants in pediatric lymphoma was similar to that in adult NK/T cell lymphoma,while the frequency of China 1,Xho I（-） or del-LMP1 in children with lymphoma was significantly higher than that in healthy donors（ all P〈0. 05）. Conclusion China 1,Xho I（-） and del-LMP1 are the major LMP1 subtypes in pediatric lymphomas from Qingdao and may associate with the susceptibility of pediatric lymphomas.

关 键 词：淋巴瘤 EB病毒 潜伏膜蛋白1 基因多态性 儿童 青岛 

分 类 号：R373[医药卫生—病原生物学]