新疆地区HLA-Cw基因多态性与宫颈癌的相关性研究  

Correlation Research between HLA-Cw Gene Polymorphism and Cervical Cancer in Xinjiang

作　　者：刘奎灿[1] 黄莉[1] 胡云辉[1] 王若峥[1] Liu Kuican Huang Li Hu Yunhui Wang Ruozheng(Radiotherapy Center of Affiliated Tumor hospital of Xinjiang Medical University, Urumqi, 830011)

机构地区：[1]新疆医科大学附属肿瘤医院放疗中心,乌鲁木齐830011

出　　处：《新疆医学》2016年第11期1361-1367,1372,共8页Xinjiang Medical Journal

基　　金：科技部国际合作项目资助(2012DFA31560)

摘　　要：目的了解新疆地区健康人群及宫颈癌(cervical cancer,CC)患者HLA-Cw等位基因分布情况,探索HLA-Cw等位基因与CC的相关性。方法选取2012-06-01至2014-06-01期间新疆医科大学附属肿瘤医院收治的308例CC患者(其中维吾尔族患者198例,汉族患者110例)及同时期204例健康人群(其中维吾尔族健康人群101例,汉族健康人群103例),采集外周血并分离提取DNA,检测HLA-Cw基因,计算各等位基因的基因表现频率,比较不同亚组人群及临床特征之间基因表现频率的差异。结果 1.健康人群及CC患者均检出13种相同HLA-Cw等位基因。2.维族健康对照组HLA-Cw05基因表现频率明显高于汉族健康对照组,差异有统计学意义(Pc<0.05);维族宫颈癌组HLA-Cw05基因表现频率明显低于维族健康对照组,差异有统计学意义(Pc<0.05);维族宫颈癌组HLA-Cw01、HLA-Cw03、HLA-Cw08基因表现频率明显低于汉族宫颈癌组,差异有统计学意义(Pc<0.05);维族宫颈癌组HLA-Cw06、HLA-Cw12基因表现频率明显高于汉族宫颈癌组,差异有统计学意义(Pc<0.05)。3.宫颈癌HLA-Cw基因表现频率在HPV16感染状态、年龄及病理类型的分布上差异无统计学意义(Pc<0.05)。结论同HLA-Cw等位基因在不同民族之间起着不同的作用,HLA-Cw05可能是维族宫颈癌保护性基因。Objective To understand the distribution of HLA-Cw allele in cervical cancer patients and healthy individuals in Xinjiang,and investigate the relationship between HLA-Cw allele and cervical cancer. Methods 308 cases of CC patients （198 cases of Uygur and 110 cases of Han Nationality） and 204 cases of healthy subjects （101 cases of Uygur and 103 cases of Han Nationality）who were treated in Xinjiang Tumor hospital from June 2012 to June 2014. Peripheral venous blood was collected,the genes were extracted,the HLA-Cw allele were checked, the performance frequency of every allele were calculated,and the difference of allele frequency among subgroups were compared. Results In healthy subjects and cervical cancer paitents groups,we detected 13 kinds of the same HLA-Cw allele. HLA-Cw05 performance frequency in Uygur healthy subjects was higher than that of Han healthy subjects,the difference were statistically significant （Pc〈0.05）. HLA-Cw05 performance frequency in Uygur cervical cancer paitents was lower than that of Han cervical cancer paitents,the difference were statistically significant （Pc〈0.05）. HLA-Cw01,03,08 performance frequency in Uygnr cervical cancer paitents were lower than that of Han cervical cancer paitents, the difference were statistically significant （Pc〈0.05）. HLA-Cw06,12 performance frequency in Uygur cervical cancer paitents was higher than that of Han cervical cancer paitents,the difference were statistically significant （Pc〈0.05）. Comparing the HLA-Cw performance frequency in different groups of HPV16 stage, age and pathologic type,there was no statistically significant difference （Pc〉0.05）. Conclusion Different HLA-Cw alleles play different roles in different ethnic groups. HLA-Cw05 may be a protective gene of cervical cancer in Uygur.

关 键 词：宫颈癌 HLA-Cw基因 表现频率 临床特征 

分 类 号：R713.4[医药卫生—妇产科学]