MHC抗原加工递呈HLA-DO基因多态性与HCV感染易感性的分子流行病学研究  

Molecular epidemiology research of MHC antigen processing machinery for HLA-DO genetic polymorphism and HCV infection susceptibilit

作　　者：路滟[1] 林凌云 周铮 谭京广[1] 曹彬[1] 许曦 谢旭[1] 梅树江[1] 

机构地区：[1]广东省深圳市疾病预防控制中心,518055 [2]广东省深圳市司法局第一强制隔离戒毒所 [3]深圳市流花医院 [4]深圳市龙岗区第二人民医院呼吸内科

出　　处：《中国实用医药》2016年第32期1-3,共3页China Practical Medicine

基　　金：深圳市医疗卫生类科研项目(项目编号:201302140)

摘　　要：目的探讨主要组织相容性复合体(MHC)抗原加工递呈相关基因HLA-DOA和HLADOB多态性与丙型肝炎病毒(HCV)易感性的关系。方法选取994例静脉注射吸毒人群,均进行检测血清中抗-HCV,将抗-HCV阴性者493例作为抗-HCV阴性组,抗-HCV阳性者501例作为抗-HCV阳性组。分析HLA-DOA和HLA-DOB基因SNPs与HCV感染易感性的关系以及环境-基因的交互作用。结果研究对象中年龄最小12岁,最大64岁,抗-HCV阳性组平均年龄(36.0±7.5)岁,抗-HCV阴性组平均年龄(34.0±7.9)岁;抗-HCV阳性组年龄略高于抗-HCV阴性组,差异有统计学意义(P<0.01)。两组性别构成比较差异无统计学意义(P=0.155>0.05);非深圳户籍人口抗-HCV阳性率(51.8%)略高于深圳户籍(40.0%),差异有统计学意义(P<0.05)。两组在文化程度上均以初中及以下为主,但在构成上比较差异有统计学意义(P<0.05),抗-HCV阴性组的高中及以上学历构成(24.3%)高于抗-HCV阳性组(17.0%)。抗-HCV阳性组谷丙转氨酶和谷草转氨酶的水平显著高于抗-HCV阴性组(P<0.01)。在吸毒人群中,HLA-DOA rs3128935 T→C和HLA-DOB rs2071469 A→G突变的改变均能显著降低个体感染HCV的风险,在调整了年龄、性别、户籍和文化程度以后,携带突变等位基因纯合子的个体发生HCV感染的可能性降低一半,OR值分别为0.32和0.50,P<0.01。结论 HLA-DOA rs3128935和HLA-DOB rs2071469突变的改变均能显著降低吸毒人群感染HCV的风险。Objective To investigate relationship between major histocompatibility complex （MHC） antigen processing machinery related HLA-DOA and HLA-DOB polymorphism and hepatitis C virus （HCV） infection susceptibility. Methods A total of 994 intravenous drug abuse people all received serum anti-HCV detection. 493 cases with negative anti-HCV were taken as negative anti-HCV＇group, and 50! cases with positive anti-HCV cases as positive anti-HCV group. Analysis was made on relationship between SNPs of HLA-DOA, HLA-DOB and HCV infection susceptibility, and reciprocal action of environment-gene. Results The youngest case aged 12 years old and the eldest case aged 64 years old, with mean age as （36.0 ± 7.5） years old in positive anti-HCV group and （34.0 ± 7.9） years old in negative anti-HCV group. Age in the positive anti-HCV group was slightly higher than the negative anti-HCV group, and the difference had statistical significance （P〈0.01）. There was no statistically significant difference of gender composition between the two groups （P=0.155〉0.05）. Migrant population without registered Shenzhen resideflce had higher positive anti-HCV rate （51.8%） than drug abuse population with registered Shenzhen residence （40.0%）, and the difference had statistical significance （P〈O.05）. Both groups had main educational level under junior high school, while their difference of composition had statistical significance （P〈0.05）. The negative anti-HCV group had higher proportion of educational level above senior high school （24.3%） than the positive anti-HCV group （17.0%）. The positive anti-HCV group had much higher alanine transaminase and aspartate aminotransferase levels than negative anti-HCV group （P〈0.05）. Changes of HLA-DOA rs3128935 T ＆ C and HLA-DOB rs2071469 A ＆ G mutation remarkably reduce individual HCV infection risk in drug abuse population. Regulation of age, gender, census register and educationlevel led to half reduced possibility of HCV infection in mutation

关 键 词：丙型肝炎病毒 遗传多态性 抗原加工递呈 感染结局 中国人群 

分 类 号：R512.63[医药卫生—内科学]