不同产地黄芩药材中黄芩苷等5种黄酮类成分含量的比较  

Content Comparison of Five Kinds of Flavonoids in Scutellariae Radix from Different Areas

作　　者：李云静[1] 张建逵[1] 王冰[1] 骆中华[1] 康廷国[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《时珍国医国药》2016年第12期2985-2988,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81173499);国家中医药管理局中医药行业科研专项(No.201407002);辽宁省中药原料药检测体系建设项目;2015年辽宁省大学生创新创业训练计划项目(No.20150761)

摘　　要：目的通过对黄芩中5个黄酮类成分含量的测定,对不同产地的黄芩药材质量进行比较。方法采用HPLC法,C_(18)色谱柱(4.6 mm×250mm,5μm),流动相为乙腈-0.5%甲酸水溶液,梯度洗脱,流速1.0ml·min^(-1),检测波长为278nm,柱温27℃,对5种黄酮类成分进行测定,并对测定结果进行差异显著性检验以及聚类分析。结果不同产地黄芩中黄酮类成分含量差异性较大,聚类分析将16批黄芩聚成两类。结论本研究表明栽培黄芩与野生黄芩药材的质量有较大差异性,前者的质量普遍高于后者。道地产区的黄芩质量不一定最好,应提高栽培技术来提高黄芩药材的质量及质量的均一性。Objective To compare the quality of Scutellaria baicalensisin different areas by determining their five kinds of fla- vonoids. Methods Five kinds of flavonoids in Scutellaria baicalensis were simultaneously determined by HPLC with C18 column （4.6 × 250mm,51m）. With a gradient program of acetonitrile - 0.5% formic acid at the flow rate of 1 mL/min, and the detection wavelength was set at 278nmwith the column temperature of 27℃. Hierarchical cluster analysis method and difference significance test of statistics method were also adopted in finally result analysis. Results Content of components varied with Scutellaria ba- icalensis in different areas, hierarchical cluster analysis classified 16 batches of Scutellariae Radix into 2 classes. Conclusion This study suggests that the quality of cultivated and wild Scutellariae Radix has great difference. The quality of the former is higher than the latter generally. The quality of Scutellariae Radixin the original production areas may not the best. The quality and the homogenicity of quality should be improved by cultivation techniques.

关 键 词：黄芩 黄芩苷 黄芩素 汉黄芩苷 汉黄芩素 千层纸素A 

分 类 号：R285.5[医药卫生—中药学]