黑樱桃番茄的离体培养及再生技术研究  

The Study of Vitro Culture and Regeneration System of Cherry Tomato

作　　者：王培军[1] 

机构地区：[1]太原学院,山西太原030012

出　　处：《太原学院学报（自然科学版）》2016年第4期61-63,共3页Journal of TaiYuan University:Natural Science Edition

基　　金：山西省星火计划项目"黑色樱桃番茄工厂化育苗技术开发"

摘　　要：文章以黑樱桃番茄的子叶、茎段、下胚轴为外植体材料,在MS中附加不同浓度激素的培养基上进行离体培养,对不定芽分化、芽的增殖培养基及不定芽生根培养基进行研究,结果表明:子叶、茎段和下胚轴均可诱导形成不定芽,但以子叶为培养材料效果最佳,最适合芽分化的培养基为MS+6-BA2.0mg/L+2,4-D0.3mg/L;芽的增殖培养基最优组合为MS+6-BA2.5mg/L+2,4-D0.2mg/L;不定芽伸长后在1/2MS培养基上生根效果最好。Callus induction and plant regeneration of cherry tomato were studied in this paper. The cotyledon, stem segments and hypocotyls of cherry tomato as the explants were cultured in tubes. The explants were cultured in MS media composing of different concentration of different plant hormones to be induced optimum shoot, proliferation of shoots and induced root. The results show that compared with stem segments and hypocotyls, cotyledon were much easier to induce shoot. The most suitable culture medium for bud differentiation was MS ＋ 6-BA 2.0mg/L ＋ 2,4-D 0.3mg/L, the proliferation medium for shoots was MS ＋6-BA2.5mg/L ＋ 2,4-D 0.2mg/L ,and for shoot growth was 1/2MS.

关 键 词：樱桃番茄 离体培养 植株再生 

分 类 号：S662[农业科学—果树学]