糖心安对Ⅱ型糖尿病大鼠周围神经病变的保护作用及其机理研究  

Study on the effect and mechanism of Tangxinan on the diabetic neuropathy in rats

作　　者：向晓雪[1] 葛麟[2] 王莉 谭正怀[2] Xiang Xiaoxue Ge Lin Wang Li Tan Zhenghuai(Sichuan College of Traditional Chinese Medicine, Miangyang 621000 Sichuan Academy of Chinese Medicine Sciences, Chengdu 6100412 Pucheng Hospital, Pucheng 715500)

机构地区：[1]四川中医药高等专科学校,绵阳621000 [2]四川省中医药科学院,成都610041 [3]蒲城县医院,蒲城715500

出　　处：《中药药理与临床》2016年第5期82-85,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：四川省科学技术厅支撑项目(2008SZ0126);成都市科学技术局支撑项目支持(09GGYB978SW-012)

摘　　要：目的:评价糖心安胶囊对Ⅱ型糖尿病致周围神经病变的防治作用及相关机制。方法:将SD雄性大鼠随机分为两组,即对照组和模型组。所有造模动物灌胃高糖高脂乳,正常对照组给等体积蒸馏水,每天一次,连续4周,然后一次性尾静脉注射链脲菌素25mg/kg以复制2型糖尿病模型。正常对照组动物给予等容积缓冲液。将造模成功大鼠随机分为5组:模型组、盐酸二甲双胍0.1 g/kg+卡托普利0.25g/kg、糖心安0.3 g/kg、糖心安0.15 g/kg、糖心安0.075 g/kg,灌胃给药,模型组和正常对照组分别给于配药溶剂0.5%羧甲基纤维素钠;每周称重,调整给药量,持续给药8周。在末次给药后24小时,测定其坐骨神经运动神经传导速度、血清TG、TC、GLU、SOD、MDA、糖化血清蛋白、AGEs,并取其坐骨神经做病理组织学检测。结果:在造型8周末其模型大鼠血清LDLC含量增加,MDA明显升高,糖化血红蛋白以及糖基化终末产物均显著增加,其坐骨神经传导速度(MNCV)降低,有髓纤维髓鞘空泡变性至严重脱髓鞘,神经纤维变细,部分神经组织局部发生肿胀现象,许旺细胞增殖等病理改变;糖心安0.3g/kg能显著降低血清中MDA、糖化血红蛋白以及AGEs,显著提高坐骨神经传导速度,改善糖尿病大鼠外周神经病理形态学变化。结论:糖心安对Ⅱ型糖尿病周围神经病变具有一定的防治作用,其作用机理可能与其能降低糖尿病大鼠血清糖化血红蛋白、糖基化终末产物以及MDA水平有关,其详细作用机理还有待进一步研究。Object： Evaluation of the effect of Tangxinan on diabetic neuropathy（ DN）. Methods： SD rats were induced diabetic peripheral neuropathy by ig high fat and sugar food and iv STZ 25 mg/kg. 3 days later,DN rats were divided into 5 groups. These rats were ig Tangxinan300 mg/kg,150 mg/kg,75 mg/kg,metformin 100 mg/kg or 0. 5% same volume of CMC respectively for 8 weeks. The levels of TG,TC,GLU,SOD,MDA,HbA 1C and AGEs in serum,the conduction velocity and pathomorphology of sciatic nerve were measured in rats. Results： After8 weeks,the levels of LDL-C,MDA,HbA 1C and AGEs in serum were significantly increased but the conduction velocity of sciatic nerve were significantly decresed in DN rats. Tangxinan0. 3g/kg can significantly reduce the levels of MDA,HbA 1C and AGEs in serum,and improve the conduction velocity and pathomorphology of sciatic nerve in DN rats. Conclusions： These results showed that the protective effect of Tangxinan on the DN may be related to lower the levels of MDA,HbA 1C and AGEs in serum.

关 键 词：糖心安 糖尿病神经病变 

分 类 号：R285.5[医药卫生—中药学]