水稻配子体雄性不育细胞质多态性及恢保关系差异研究  

A Study of Cytoplasmic Polymorphism and Restore-maintain Relationship of Rice Gametocyte Male Sterility

作　　者：钱明娟 谢红卫 蔡怡聪[2] 潘境涛 谢启鑫 李永辉 毛凌华 蔡耀辉 QIAN Ming-juan XIE Hong-wei CAI Yi-cong PAN Jing-tao XIE Qi-xin LI Yong-hui MAO Ling-hua CAI Yao-hui(Jiangxi Super-rice Research and Development Center/National Engineering Laboratory of Rice （ Nan- chang）, Nanchang 330200 China National Rice Research Institute, Hangzhou 310006 Nanchang Universi- ty , Nanchang 330031)

机构地区：[1]江西省超级水稻研究发展中心/国家水稻工程实验室(南昌),江西南昌330200 [2]中国水稻研究所,浙江杭州310006 [3]南昌大学生命科学学院,江西南昌330031

出　　处：《江西农业大学学报》2016年第6期1017-1022,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31360332);江西省科技计划重大项目(20143ACF60008);江西省科技支撑计划(20123BBF60187);江西省农业科学院博士启动基金项目(20141CBS001)~~

摘　　要：为解释育种实践中常出现的相同类型不育系与不同恢复系杂交组合F1代育性恢复有明显差异的现象,笔者通过对同核异质的5个配子体不育系W15A、W20A、W34A、W46A和YA线粒体DNA进行RAPD扩增聚类,不育基因orf(H)79基因及基因间区序列测序,测配F1分析其恢保关系,展示同型细胞质的差异与恢保差异的关系。结果表明,通过RAPD扩增聚类,这5个配子体细胞质雄性不育系在线粒体基因组可聚分为3类,而W34A与其他4个不育系的细胞质亲源关系较远。不育基因及基因间区序列均存在多个差异位点,5个水稻配子体雄性不育细胞质在大部分水稻材料的测恢中,恢保关系一致,而一部分材料的恢保关系差异较大。该研究为新型配子体不育系的选育、组合配制和同核多胞质混合杂交水稻亲本的选择提供一定的理论依据。In order to illuminate the fertility differences in the F1 generations from crossbreeding between the same type CMS lines with different restorers,five homonucleus-alloplasm Gametophyte cytoplasmic male sterile lines were used,they were W15 A,W20A,W34 A,W46A and YA.RAPD（ Random Amplified Polymorphic DNA） amplification,sequencing and crossbreeding were used to display the cytoplasmic diversity and the various restore-maintain relationships.Based on RAPD polymorphism amplification of mitochondrial DNA,the five CMS lines could be divided into three categories,W34 A had the furthest relationship with the others. Via sequence analysis of the orf（ H） 79 gene and the linked segment showed nucleotide variations.Fertility analysis of F1 generations from crossing between the five CMS lines with different restorers showed that most restore-maintain relationships were identical,but differences in several hybridized combinations were also detected.Therefore,the study could provide theoretical guidance for breeding new sterile cytoplasm,hybrid combination design and selection of rice parents to get homonucleus-alloplasm cultivars.

关 键 词：配子体细胞质雄性不育 多态性 线粒体基因组 水稻 

分 类 号：S511.032[农业科学—作物学]