先天性EDNRB基因缺陷对大鼠海马区神经细胞增殖的影响  

The effection of neural proliferation in the hippocampal formation in rats modal with congenital EDNRB defect

作　　者：谢聃[1] 宋赞民 李继梅[1] 

机构地区：[1]首都医科大学附属北京友谊医院神经内科,北京100050 [2]澳大利亚国立大学约翰科廷医学研究中心神经研究所

出　　处：《临床和实验医学杂志》2017年第2期114-117,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的选取先天性EDNRB基因缺陷大鼠,通过与野生型及杂合子大鼠相比较,探讨先天性内皮素受体B(EDNRB)缺陷对大鼠海马区神经细胞增殖的影响。方法采用EDNRB基因缺陷大鼠模型,将新生大鼠在出生后2天时对其进行RTQ-PCR基因检测,并根据检测结果分为杂合子组(+/sl)纯合子组(sl/sl)及野生组(+/+);所有幼鼠组在出生后第2天行经磷酸缓冲盐溶液心脏灌注后取脑组织标本,注射Brdu后2 h行心脏灌注取脑组织。将脑组织标本固定后冰冻切片,对切片后的脑组织选取海马进行Brdu染色,并在镜下观察增殖细胞数,对结果进行统计分析。结果EDNRB基因缺陷组海马CA1区、CA3区及齿状回的神经细胞增殖较杂合子组及野生基因组明显减少(P<0.01)。结论EDNRB基因缺陷的大鼠,与杂合子及野生鼠相比,其脑组织海马CA1、CA3区及齿状回均发现新生细胞增殖的减少。Objective To set up a congenital EDNRB defect rat model,and to investigate the effect of EDNRB defect in the neural proliferation in hippocampus.Methods This experiment use the congenital endothelin receptor（EDNRB） genetic defect model rat,RTQ- PCR genetic testing would be used two days after birth.According to the results of genetic testing,all rats divided into heterozvgote groups（＋ /sl）,homozygous group（sl/sl） and wild group（＋/＋）;2 days after birth,brain tissue specimens will be gained after the menstruation phosphate buffer solution heart perfusion.The brain tissue will be collected after the injection of Brdu 2 h.Brain section will be frozen,after tissue specimens and fixed on the slice.The cell proliferation in hippocampal section was detected under the microscopic.The results were statistically analyzed.Results Spotting lethal rat has reduced neural proliferation in hippocampal formation,including area CA1,CA3 and dentate gyrus.Conclusion Compared with the heterozygote and wild rats,the neural proliferation were decreased in hippocampus area CA1,CA3 and dentate gyrus in rat with EDNRB gene defect.

关 键 词：大鼠 先天性内皮素受体B缺陷 齿状回 海马 细胞增殖 先天性巨结肠症 

分 类 号：R725.7[医药卫生—儿科]