杨梅素通过上调PHLPP1的表达抑制A549细胞迁移  

Myricetin Inhibited the Migration of A549 Cells by Up-regulating PHLPP1

作　　者：苏丽娟[1] 付有荣[1] 田莉[1] 陈明[1] 

机构地区：[1]武汉大学中南医院输血科,湖北武汉430071

出　　处：《临床和实验医学杂志》2017年第2期131-134,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的探究杨梅素通过上调PHLPP1表达从而抑制A549细胞迁移的机制。方法体外培养人A549细胞,分别用溶剂DMSO、10μmol/L杨梅素、20μmol/L杨梅素、40μmol/L杨梅素和80μmol/L杨梅素处理细胞24 h,MTT法检测杨梅素对A549细胞增殖的抑制情况;RNA干扰技术敲降细胞内PHLPP1蛋白的表达;Western blot实验检测空白对照组、阴性对照组和PHLPP1敲降组的PHLPP1蛋白表达情况;transwell迁移实验检测各组细胞的迁移情况。结果MTT结果显示,与0μmol/L组相比,20μmol/L、40μmol/L和80μmol/L的杨梅素可以显著抑制A549细胞增殖(P<0.01);transwell迁移实验结果显示,与0μmol/L组相比,20μmol/L和40μmol/L的杨梅素能显著抑制A549细胞迁移(P<0.01);当PHLPP1被敲降后,与空白对照组和阴性对照组相比,20μmol/L杨梅素抑制A549细胞迁移的能力显著减弱(P<0.01)。结论杨梅素可以通过上调PHLPP1的表达抑制A549细胞迁移。Objective To explored the mechanism that myricetin inhibited the migration of A549 cells by up- regulating PHLPPl.Methods A549 cells were treated with DMSO,10μmol/L myricetin,20μmol/L myricetin,40μmol/L myricetin,80μmol/L myricetin for 24 hours.The MTT assay was used to detect the proliferation of A549 cells.The RNA interference was used to knock down the expression of PHLPPl.Western blot assay was used to detect the expression of PHLPPl in blank group,vector group and PHLPPl knocked- down group.Transwell migration assay was used to detect the migration of A549 cells.Results Compared with 0 μmol/L group,Myricetin inhibited the proliferation of A549 cells at the concentration of 20μmol/L,40μmol/L and 80μmol/L（P 〈0.01）.Compared with 0μmol/L group,Myricetin inhibited the migration of A549 cells and up- regulated the expression of PHLPPl at the concentration of 20 μmol/L and 40 μmol/L（P 〈0.01）.After PHLPPl was knocked down,compared with blank group and vector group,the migratory inhibiting effect of 20 μmol/L myricetin was alleviated in A549 cells（P〈 0.01）.Conclusion Myricetin can inhibite the migration of A549 cells by up- regulating PHLPPl.

关 键 词：杨梅素 PHLPP1 A549 迁移 

分 类 号：R285[医药卫生—中药学]