解毒活血中药组分复方对脂多糖诱导巨噬细胞炎症反应的抑制作用  

Inhibition of Ingredients in Jiedu Huoxue Chinese Medicine Compound on Macrophage Inflammatory Response Induced by LPS

作　　者：刘钊[1] 钟菊迎[1] 高尔宁 杨鸿[1] 

机构地区：[1]中国中医科学院医学实验中心,北京100700

出　　处：《中国实验方剂学杂志》2017年第1期25-29,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院自主选题项目(ZZ2012001;ZZ2015008)

摘　　要：目的:优选抑制过度免疫反应的解毒活血中药组分复方(栀子苷、川芎嗪和甘草酸),为此类疾病的治疗提供参考。方法:将栀子苷、川芎嗪和甘草酸质量浓度3个因素按9个水平数进行均匀设计,应用液相蛋白芯片技术检测解毒活血中药组分复方不同配伍水平对脂多糖诱导小鼠巨噬细胞RAW264.7炎症模型表达的23种细胞因子水平的影响。使用"中药组方优化平台"V1.0分析复方中各组分质量浓度和细胞因子抑制率间的量效关系,获得抑制过度炎症反应效应最佳的中药组分复方并对优化配伍进行验证。结果:栀子苷-川芎嗪-甘草酸(1∶20∶19)的预测复方对脂多糖引起的巨噬细胞过度炎症反应的综合抑制率最高,综合抑制率预测值和试验获得值分别为43.3%和28.3%。结论:运用均匀设计与药效学验证相结合的方法可筛选出具有更佳抗炎活性的新中药组分复方。Objective： To optimize ingredients in Jiedu Huoxue Chinese medicine compound（geniposide,ligustrazine and glycyrrhizic acid） for inhibiting excessive immune response. Method： In the study,uniform design was adopted to design doses of geniposide, ligustrazine and glycyrrhizic acid, the liquichip technique was used to detect the effect of different compatibility level on expression of 23 kinds of cytokines in RAW264. 7 inflammation model induced by lipopolysaccharide（LPS）,with the comprehensive weight method,the traditional Chinese medicine component optimization software and the improved multiple linear regression algorithm were used to produce the regression equation and the optimal dose ratio of geniposide,ligustrazine and glycyrrhizic acid, through analyzing the dose-effect relationship among the three components and the cytokine inhibition rate. Result： When the ratio of geniposide, ligustrazine and glycyrrhizic acid was 1 ∶ 20 ∶ 19, the combination had the best anti-inflammatory effects in RAW264. 7 inflammation model induced by LPS, its predicted and experimentally obtained values of comprehensive inhibition rate were 43. 3% and 28. 3%.Conclusion： The combination of uniform design and cell models in pharmacodynamics is an effective way to optimize the proportion of Chinese medicinal components.

关 键 词：中药组分复方 细胞因子 均匀设计 综合权重法 抗炎 脂多糖 

分 类 号：R945[医药卫生—微生物与生化药学] R285.5[医药卫生—药剂学]