碳酸饮料对雄鼠生长发育和睾酮分泌作用的研究  

Effects of Carbonated Drinks on Growth and Testosterone Secretion of Male Mice

作　　者：巩转娣[1] 魏锁成[2] 魏黎敏[3] 张涛杰[2] 杨妍梅[2] 梁浩勤 王欢[2] 赖露菊 郑秧秧 

机构地区：[1]西北民族大学医学院附属医院,甘肃兰州730030 [2]西北民族大学生命科学与工程学院,甘肃兰州730030 [3]甘肃省人民医院神经内科,甘肃兰州730099

出　　处：《食品与生物技术学报》2016年第11期1163-1168,共6页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31460684)

摘　　要：将100只28日龄健康雄性小白鼠随机分为5组(n=20),COC-1和COC-2自由饮用50%和100%可口可乐,PEP-1和PEP-2自由饮用50%和100%百事可乐,CG为空白对照,实验持续15 d。于第3、5、7、10、13和15 d随机从每个组抓取3只小鼠断颈采血,4 000 r/min离心10min,分离血清,无菌采集内脏器官,分别称重;ELISA法测定血清睾酮含量。结果表明至实验第7 d,COC-2和PEP-2组小鼠的体重均高于CG(P<0.01,P<0.05),15 d时,实验组小鼠体重均大于对照组(CG);第10 d时COC-1日增重明显低于PEP-2和CG,第15 d各实验组日增重均略小于对照组;第7 d时,COC-1、COC-2和PEP-2两侧睾丸平均重明显高于CG(P<0.05),到15d实验组睾丸重均大于对照组(CG);PEP-2胃质量在第15 d大于其余4组;第7 d时COC-1、COC-2和PEP-2肾脏质量明显高于PEP-1(P<0.05);在实验期间各组的心、肝、脾和肺脏均无显著差异;第10d时PEP-1,PEP-2血清睾酮含量显著高于COC-1和CG(P<0.05),且COC-2和PEP-2在15 d明显高于CG(P<0.05)。短时期饮用高浓度可口可乐和百事可乐可以促进小鼠生长,轻度促进睾丸发育,增加血清睾酮含量。One hundred male mice （20.84±2.45g） were randomly divided into five groups （n=20）, COC-1 and COC-2 groups drank ad libitum 50% and 100% Coca-cola, PEP-1 and PEP-2 mice drank 50% and 100% Pepsi-cola and the control group （CG） drank tap water. Three mice selected randomly fi＇om each group were killed by cervical dislocation on the days 3,5,7,10,13 and 15;the serum was separated by centrifugal at 4000r/min for 10min; all visceral organs （heart, liver, spleen, lung, kidney, stomach and testis ）were harvested aseptically ;the concentration of serum testosterone was measured with the ELISA kit. On the 7th day body weights of four test groups were all higher than that of CG ,especially for COC-2 （P〈 0.05）;on the 15th day the body weights of COC-2 and PEP-2 groups were higher than that of CG. Testis weights of COC-1, COC-2 and PEP-2 groups were significantly greater than groups CG and PEP-1 on the 7th day （P〈0.05）;the stomach weight of PEP-2 was higher than other four groups on the 15th day. The concentration of serum testosterone in PEP-2 was significantly higher than those in COC-1 and CG （P 〈 0.05）;on the 10th day the concentrations in COC-2 and PEP-2 were dramatically higher than that in CG （P 〈 0.05 ） ;on the 15th day the concentration in PEP-2 was remarkably higher than that in CG （P 〈0.05）. Thus,a short period of drinking of relatively high concentrations of Coca-cola and Pepsi-cola could promote the growth of male mice, enhance the testis development and increase the content of serum testosterone with no remarkable effects on visceral organs.

关 键 词：碳酸饮料 生长发育 生殖功能 睾酮 小鼠 

分 类 号：TS275.3[轻工技术与工程—农产品加工及贮藏工程]