SIRT1基因沉默对高脂诱导的C57BL/6J小鼠肝脏影响  

Effect of SIRT1 gene silencing on liver tissue of high-fat-induced C57BL/6J mice

作　　者：高英[1] 陈晓媛[1] 杨怡[2] 

机构地区：[1]宁夏医科大学总医院儿科,宁夏银川750004 [2]宁夏医科大学,宁夏银川750004

出　　处：《宁夏医学杂志》2016年第12期1105-1107,I0001,共4页Ningxia Medical Journal

基　　金：国家自然科学基金资助项目(81670798)

摘　　要：目的初步探讨SIRT1基因沉默对高脂喂养C57BL/6J幼鼠肝脏的影响。方法选取4周龄的雄性C57BL/6J小鼠32只,分为4组:高低脂饮食组及慢病毒注射组。在高脂饮食喂养6周后,尾静脉分别注射SIRT1shRNA慢病毒(LV-SIRT1 shRNA)和对照慢病毒(LV-control shRNA),继续高脂饮食1周。Western Blot检测肝脏中SIRT1表达情况,HE和油红O染色观察肝脏脂滴堆积情况。结果 Western Blot显示LV-SIRT1 shRNA慢病毒小鼠肝组织中SIRT1表达水平显著下降;肝组织HE和油红O染色显示,LV-SIRT1 shRNA组较LV-control shRNA组脂肪肝更明显。结论慢病毒介导SIRT1基因沉默可以加重高脂饮食诱导的非酒精性脂肪肝风险。Objective To investigate the effect of SIRT1 gene silencing on the liver tissue of high - fat ：- induced C57BL/6J im- mature rats. Methods The male C57BL/6J mice （4 -old -week,n =32） were selected and divided into low -fat diet （LFD） ,high -fat diet （HFD） and lentivirus - injected groups. After all of them were HFD - fed for 6 weeks, the LV - SIRT1 shRNA or LV - control shRNA were injected into the tail vein of these I-IFD -fed mice separately,then the mice were HFD -fed for 1 week continously. Then the SIRT1 protein expression level of liver was detected by western blotting. And the accumulation of liver morphology and lipid droplets in liver were observed by H＆E or Oil red O staining. Results Western blotting showed that SIRT1 expression level was significantly decreased in the LV - SIRT1 shRNA group. The H＆E or Oil red O staining showed that comparing with the LV - control shRNA group, the level of lipid droplets synthesis and steatosis in the LV - SIRT1 shRNA group significantly decreased. Conclusions Lentivirus - mediated SIRTI gene silencing could aggravate the risk of non - alcoholic fatty liver disease （NAFLD） induced by HFD.

关 键 词：SIRT1 基因沉默 高脂 非酒精性脂肪肝 

分 类 号：R723.14[医药卫生—儿科]