人CITED4基因及蛋白的生物信息学分析  

Bioinformatic analysis of CITED4 gene and protein of human

作　　者：王媛[1] 韩如意[1] 苏玉贞[1] 孙丽[1] 张连民[1] 王晨[1] 

机构地区：[1]天津医科大学肿瘤医院,国家肿瘤临床医学研究中心,天津市肿瘤防治重点实验室,天津300060

出　　处：《生物技术》2016年第6期566-573,共8页Biotechnology

基　　金：国家自然科学基金项目(“mTOR调控P62降解在胶质瘤自噬中的作用及其机制研究”,No.81602213)

摘　　要：[目的]预测人CITED4基因启动子信息及其蛋白质的理化性质、亲疏水性、细胞亚定位、蛋白质结构、相互作用蛋白质以及GO注释，以期为发现其新功能提供理论和结构基础。[方法]利用PromoterScan、ProtParam和ClustalX2．1等预测分析CITED4基因及其蛋白的相关信息。[结果]CITED4基因有2个启动子，其转录活性受SP1、AP-2和CREB影响；其蛋白质是由184个氨基酸组成的主要定位于细胞核的不稳定疏水蛋白质，不稳定系数为65．05；氨基酸序列在152～173间保守性强；二级结构含有47个d螺旋16个B折叠；三级结构的建立需要更多可靠的模板，CITED4通过与AP-2等转录因子相互作用调控多个基因的转录活性。[结论]CITED4表达受SP1、AP-2和CREB的调控，CITED4蛋白通过调节多个靶基因的转录调控人体正常生理或病理状态。[ Objective ] Analyzing the promoter, physicochemical property, hydrophilcity/hydrophobicity, subcellar location, protein structure, protein - protein interaction and Gene Ontology provide the theoretical and structural basis for the discovery of the new function of CITED4. [ Methods ] By meaning of Promoter Scan, ProtParam and ClustalX2.1, the messages of CITED4 are predicted and analyzed. [ Results ] CITED4 gene has two promoters, and its transcriptional activity regulated by SP1, AP - 2 and CREB. CITED4 protein was composed of 184 amino acids,which was unstable,hudrophobinand and located in nucleus. The protein was highly conservative between 152 and 173; the secondary structure contains 47 α -helices and 16 [3 -sheets; and the three -fold structure of the protein need to be more reliable template,which are mainly composed of CITED4 regulates the transcriptional activity of multiple genes by interacting with AP - 2 and other transcription factors. [ Conclusion ] The expression of CITED4 regulated by SP1, AP -2 and CREB. CITED4 mainly located in nucleus and participated in the human normal physiologic or pathological condition by regulating several gene transcription and expression.

关 键 词：C1TED4 AP-2 基因转录 生物信息学 

分 类 号：Q811.4[生物学—生物工程]