喷托维林对肝癌HepG2细胞增殖和凋亡的影响  

Effect of pentoxyverine on HepG2 cell proliferation and apoptosis

作　　者：熊明涛 占劲松 朱吉生[1] 邵金辉[2] 

机构地区：[1]湖北文理学院医学院附属谷城县人民医院,湖北襄阳441700 [2]湖北文理学院医学院形态学部,湖北襄阳441053

出　　处：《生物技术》2016年第6期602-605,共4页Biotechnology

基　　金：湖北省教育厅科学研究计划指导性项目(No.B2015144);谷城人民医院横向课题;湖北文理学院博士科研启动基金项目(No.2013B009)

摘　　要：[目的]研究枸橼酸喷托维林对肝癌HepG2细胞增殖和凋亡的影响。[方法]采用不同浓度(60、75和90μmol/L)的枸橼酸喷托维林处理HepG2细胞48 h,应用MTT(噻唑蓝)法测定细胞的增殖活性,再检测乳酸脱氢酶(LDH)活性,Hoechst 33258染色法检测细胞的凋亡情况。[结果]枸橼酸喷托维林能以浓度依赖的方式抑制HepG2细胞的增殖。对照组与处理组之间LDH活性比较均无显著性差异(P>0.05)。而75μmol/L枸橼酸喷托维林处理48 h能够显著诱导HepG2细胞凋亡。[结论]枸橼酸喷托维林可显著抑制肝癌HepG2细胞增殖,这与其诱导细胞凋亡途径有关。[ Objective ] To find out whether pentoxyverine citrate can inhibit hepatic cancer （HepG2 cell） and to explore the inhibition mechanism. [ Methods ] The HepG2 cells have been treated by different concentrations （60,75 and 90 μmol/L）of pentoxyverine citrate for 48 h. Then the proliferative activity was determined by the MTT assay, and the apoptosis was determined by Hoechst 33258 staining assay. Lactate dehydrogenase （ LDH ） was also tested. [ Results ] The proliferation of the HepG2 ceils were significantly suppressed by the drug in a dose - dependent manner. However, there were no significant differences （ P 〉 0.05 ） in the LDH release of the ceils between the control group and the drug treatment groups. Significant apoptosis was induced in HepG2 cells after incubated with 75 μmol/L of pentoxyverine citrate for 48 h. [ Conclusion] Pentoxyverine citrate could inhibit the proliferation of HepG2 cells by inducing cellular apoptosis. The drug may be a clue to find effective and safe drug for heptic cancer.

关 键 词：喷托维林 HEPG2细胞 增殖 细胞凋亡 

分 类 号：R979.19[医药卫生—药品]