两种内参基因在鸡循环miRNA相对定量检测中的比较分析  

Compared Analysis of Two Control Genes in Chicken Circulating miRNA RT-qPCR Test

作　　者：朱云芬[1] 韩威[1] 苏一军[1] 李国辉[1] 王洪志[1] 张会永[1] 殷建玫[1] ZHU Yunfen HAN Wei SU Yijun LI Guohui WANG Hongzhi ZHANG Huiyong YIN Jianmei(Poultry Institute,Chinese Academy of Agrieultural Seiences,Yangzhou,Jiangsu 225125)

机构地区：[1]中国农业科学院家禽研究所,江苏扬州225125

出　　处：《中国家禽》2016年第24期18-21,共4页China Poultry

基　　金：国家自然科学基金项目(31201799);江苏省农业三新工程项目(SXGC(2015)300);江苏省农业科技创新项目(CX(15)1009)

摘　　要：选择5SrRNA和snRNAu6作为内参基因，比较分析其在鸡血清循环miRNA定量检测中的应用效果。结果显示，5SrRNA和snRNAu6两个内参基因在不同发育阶段血清中表达稳定，5SrRNA的Cq值在24．4～25．0之间，snRNAu6的cq值在24．0-24．5之间，组间Cq值的变化幅度小于0．6；同一测定miRNA的组内Cq值变异也较小。两种内参基因条件下，3个测定循环miRNA的表达变化规律均一致。表明5SrRNA和snRNAu6是适于鸡血清循环miRNA定量检测的内参基因。Two control genes,SS rRNA and snRNA U6,were selected to compare the effects in chicken （G. gallus） serum circulating miRNA real-time quantitative PCR （RT-qPCR） tests. The results showed that expression levels of 5 S rRNA and snRNA U6 were relatively stable between different groups, with 24.4 to 25.0 Cq values for 5 S rRNA and 24.0 to 24.5 Cq values for snRNA U6. The within-group Cq values for each tested miRNA also had less variation. The tested three miRNAs had the same expression tendency using either 5 S rRNA or snRNA U6. The results indicated that 5 S rRNA and snRNA U6 were suitable control genes for chicken serum circulating miRNA RT-qPCR test.

关 键 词：内参基因 微小RNA 相对定量 鸡 

分 类 号：S831.2[农业科学—畜牧学]