增效醚对二化螟幼虫7-乙氧基香豆素-O-脱乙基酶和羧酸酯酶活性的影响  

In vivo effects of piperonyl butoxide on the activities of 7-ethoxycoumarin O-deethylase and carboxylesterase in larval Chilo suppressalis( Lepidoptera:Pyralidae)

作　　者：李阳[1] 史雪岩[1] 高希武[1] LI Yang SHI Xue-Yan GAO Xi-Wu(Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China)

机构地区：[1]中国农业大学植物保护学院昆虫系,北京100193

出　　处：《昆虫学报》2016年第11期1159-1165,共7页Acta Entomologica Sinica

基　　金：公益性行业(农业)科技专项(201203038)

摘　　要：【目的】通过研究二化螟Chilo suppressalis细胞色素P450酶活性特征及增效醚(piperonyl butoxide,PBO)对二化螟幼虫7-乙氧基香豆素-O-脱乙基酶(ECOD)和羧酸酯酶(Car E)活性影响的时间效应,为有效进行二化螟的防治及抗药性治理提供基础的研究数据。【方法】采用以7-乙氧基香豆素为底物的荧光比色法,测定了不同龄期二化螟ECOD活性,并研究了经PBO(0.69μg/头)点滴处理后24 h内每隔1 h二化螟4龄幼虫ECOD及Car E活性的变化。【结果】二化螟的ECOD活性从1龄幼虫至蛹期呈现出先升高后降低的变化趋势,其中ECOD活性在4龄幼虫期升至最高值14.11±1.01 pmol/mg pro·min,而在蛹期降到最低值(1.34±0.11 pmol/mg pro·min)。PBO处理对二化螟4龄幼虫ECOD活性的影响具有随时间而变化的抑制-诱导-抑制的动态效应:在处理后4-9 h,PBO对ECOD活性具有抑制作用,其中在处理后6 h时的抑制作用最强,抑制率达到21%;在处理后10-11 h,PBO诱导了ECOD活性升高;在处理后15-17 h,又表现为抑制作用。PBO处理二化螟后10-12 h和18-20 h对二化螟的Car E活性具有诱导作用,而在14-17 h和20-21 h表现为抑制作用。【结论】不同龄期二化螟幼虫及蛹的ECOD活性存在一定差异。PBO点滴处理对二化螟4龄幼虫的ECOD和Car E活性具有时间依赖的抑制或诱导效应。【Aim】In order to provide data for efficient management of insecticide resistance of Chilo suppressalis（ Walker）, we measured the cytochrome P450-mediated 7-ethoxycoumarin-O-deethylase（ ECOD） activity in different instars of larval C. suppressalis,and evaluated the time-dependent in vivo effects of piperonyl butoxide（ PBO） treatment on the activities of ECOD and carboxylesterase（ Car E） in the 4th instar larvae. 【Methods 】By using 7-ethoxycoumarin as the substrate,the ECOD activity in different instar larvae and pupae of C. suppressalis was determined by fluorospectrophotometric analysis.Variations of ECOD and Car E activities in the 4th instar larvae were examined at each hour within 24 hours after treatment topically by PBO（ 0. 69 μg/larva）. 【Results】The ECOD activity in C. suppressalis from the 1st instar larval stage to the pupal stage showed a firstly increasing and then decreasing trend.The ECOD activity increased to the highest level of 14. 11 ± 1. 01 pmol/mg pro·min in the 4th instar larvae,while decreased to the lowest（ 1. 34 ± 0. 11 pmol/mg pro · min） in pupae. PBO exhibited timedependent effects on the ECOD activity of the 4th instar larvae. As compared with the untreated control,PBO treatment inhibited the ECOD activity during 4-9 h with a maximal inhibition rate of 21% at 6 h,induced its activity during 10-11 h,and then inhibited its activity during 15-17 h. In addition,PBO exposure also affected the Car E activity in the 4th instar larvae,showing inhibition effects during 14-17 h and 20-21 h and induction effects during 10-12 h and 18-20 h after treatment. 【Conclusion】The ECOD activity varies in different instar larvae and pupae of C. suppressalis. PBO treatment displays time-dependent effects of inhibition or induction on the activities of ECOD and Car E in the 4th instar larvae of C. suppressalis.

关 键 词：二化螟 增效醚 细胞色素P450酶 7-乙氧基香豆素-O-脱乙基酶 羧酸酯酶 

分 类 号：Q965.9[生物学—昆虫学]