斜纹夜蛾抗药性监测及茚虫威对其解毒代谢酶的影响  

Monitoring of insecticide resistance in Spodoptera litura( Lepidoptera:Noctuidae) and the effect of indoxacarb on its detoxification enzymes

作　　者：刘佳[1] 周勇[1] 朱航[1] 马海昊[1] 邓希乐[1] 周小毛[1] 柏连阳[2] LIU Jia ZHOU Yong ZHU Hang MA Hai-Hao DENG Xi-Le ZHOU Xiao-Mao BAI Lian- Yang(Bioteehnology Research Center, Hunan Academy of Agricultural Sciences, Changsha 410125, China Hunan Academy of Agricultural Sciences, Changsha 410125, China)

机构地区：[1]湖南省农业科学院生物技术研究中心,长沙410125 [2]湖南省农业科学院,长沙410125

出　　处：《昆虫学报》2016年第11期1254-1262,共9页Acta Entomologica Sinica

基　　金：公益性行业(农业)科研专项(201203038);国家自然科学基金项目(31371966)

摘　　要：【目的】斜纹夜蛾Spodoptera litura（Fabricius）是主要的农业害虫之一。本研究旨在明确该害虫在湖南省5个主要蔬菜种植区的抗药性水平,并探讨该害虫对茚虫威的抗性与解毒代谢酶活性之间的关系,为斜纹夜蛾有效防控及抗性治理提供依据。【方法】采用浸叶法测定了2014-2016年湖南5地斜纹夜蛾田间种群对10种杀虫剂的抗性水平;将斜纹夜蛾敏感种群3龄幼虫在死亡率40%-70%的选择压下用茚虫威进行汰选,比较了斜纹夜蛾敏感种群和抗茚虫威种群的羧酸酯酶、谷胱甘肽S-转移酶和多功能氧化酶对硝基苯甲醚O-脱甲基活性。【结果】湖南5地斜纹夜蛾田间种群对有机磷类杀虫剂产生了26.9-220.2倍的抗性,对氨基甲酸酯类杀虫剂产生了68.3-890.8倍的抗性,对拟除虫菊酯类杀虫剂产生了21.0-267.2倍的抗性,对相对较新型杀虫剂（甲维盐、阿维菌素、茚虫威和溴虫腈）产生了5.2-53.4倍的抗性。经茚虫威汰选后第14代[抗性倍数（resistance ratio,RR）=26.43]斜纹夜蛾羧酸酯酶、谷胱甘肽S-转移酶和对硝基苯甲醚O-脱甲基酶活性分别上升2.86,1.01和1.83倍。【结论】斜纹夜蛾对多种药剂产生了不同水平的抗性,斜纹夜蛾幼虫羧酸酯酶和对硝基苯甲醚O-脱甲基活性增强可能是斜纹夜蛾对茚虫威的抗性上升的重要因素。【Aim】The common cutworm,Spodoptera litura（ Fabricius）,is one of major agricultural pests all over the world. In this study we evaluated the resistance level of S. litura to insecticides in five major vegetable growing areas of Hunan province and determined the relationship between the resistance to indoxacarb and the activities of detoxification enzymes in S. litura,aiming to offer the theoretical basis for the control of this pest. 【Methods】The resistance to 10 insecticides in S. litura populations from five districts of Hunan Province from 2014 to 2016 was assayed using a standard leaf dip bioassay method.Indoxacarb was used to select a resistant strain under 40%-70% selection pressure. The activities of Car E,GST and PNOD in the 3rd instar larvae of the susceptible and indoxacarb-resistant strains were tested. 【Results】Compared with the susceptible strain,the resistant strain of S. litura obtained 26. 9-220. 2-fold resistance to organophosphates,68. 3-890. 8-fold resistance to carbamates,21. 0-267. 2-fold resistance to pyrethroids and 5. 2-53. 4-fold resistance to relatively novel insecticides including emamectin,abamectin,indoxacarb and chlorfenapyr. After 14 continuous generations of selection with indoxacarb,the selected population obtained 26. 43-fold resistance compared with its unselected population. The activities of Car E,GST and PNOD in the selected strain were 2. 86,1. 01 and 1. 83-fold higher than those in the unselected strain,respectively. 【Conclusion】S. litura has developed different levels of resistance to the tested insecticides,and the enhancement in the activities of Car E and PNOD is an important mechanism for the increased resistance of S. litura to indoxacarb.

关 键 词：斜纹夜蛾 抗药性 茚虫威 解毒酶系 代谢抗性 

分 类 号：Q965.9[生物学—昆虫学]